Modular Assembly of Glycoproteins: Towards the Synthesis of GlyCAM-1 by Using Expressed Protein Ligation

Put the two together: Physiologically relevant glycoproteins containing saccharides at predetermined sites, such as GlyCAM‐1, can be generated from small synthetic and bacterially derived fragments by using the expressed protein ligation methodology. This technique was used for the synthesis of thre...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2004-03, Vol.43 (11), p.1355-1359
Main Authors: Macmillan, Derek, Bertozzi, Carolyn R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
expressed protein ligation
glycopeptides
Glycopeptides - chemical synthesis
Glycopeptides - chemistry
glycoproteins
Glycoproteins - chemical synthesis
Glycoproteins - chemistry
Glycosylation
Ligands
Molecular Sequence Data
Mucins - chemical synthesis
Mucins - chemistry
protein engineering
Protein Structure, Tertiary - physiology
semisynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Put the two together: Physiologically relevant glycoproteins containing saccharides at predetermined sites, such as GlyCAM‐1, can be generated from small synthetic and bacterially derived fragments by using the expressed protein ligation methodology. This technique was used for the synthesis of three semisynthetic GlyCAM‐1 variants 1–3 (see scheme).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200352673