Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail

A β- d-glycosidase (G I) from the China white jade snail showed non-Michaelis–Menten mode in catalyzing the reaction using pNPGlu and pNPFuc as the substrate and monitoring the released pNP. We determined quantitatively both the transglycosidic and hydrolytic products of pNPGlu and pNPFuc solvolysis...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biotechnology 2009-02, Vol.139 (3), p.229-235
Main Authors: Hu, Ying, Luan, Hongwei, Ge, Guangbo, Liu, Huixin, Zhang, Yanyan, Zhou, Kun, Liu, Yong, Yang, Ling
Format: Article
Language:English
Subjects:
1-Deoxynojirimycin - pharmacology
Allosteric effect
Animals
Biological and medical sciences
Biotechnology
Butanols - metabolism
Cellobiose - metabolism
Fundamental and applied biological sciences. Psychology
Glucosamine - analogs & derivatives
Glucosamine - pharmacology
Glycoside Hydrolases - metabolism
Glycosides - metabolism
Glycosylation
Hydrolysis
Kinetics
Nonlinear Dynamics
Regression Analysis
Snails - enzymology
Substrate Specificity
The China white jade snail
Transglycosylation activity
β- d-Glycosidase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A β- d-glycosidase (G I) from the China white jade snail showed non-Michaelis–Menten mode in catalyzing the reaction using pNPGlu and pNPFuc as the substrate and monitoring the released pNP. We determined quantitatively both the transglycosidic and hydrolytic products of pNPGlu and pNPFuc solvolysis for the detailed kinetic analysis on G I-catalyzed hydrolysis and transglycosylation reaction. The inhibition kinetic studies using deoxynojirimycin (DNJ) and butanol as inhibitors were preceded. DNJ only inhibited competitively the hydrolysis of cellobiose and pNPGlu while “activated” the transglycosylation of pNPGlu and pNPFuc. This was evident from the increased V max tr value with no change of the apparent K m tr. In contrast, butanol exhibited a competitive inhibition to the transglycosylation reaction and non-competitive inhibition to the hydrolysis. The results indicated that the non-Michaelis–Menten kinetic behavior was caused by the co-occurrence of substrate transglycosylation reaction. This study provided a simple method to increase the transglycosylation yield by using DNJ to inhibit hydrolysis.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2008.12.006