A simple and sensitive HPLC–ESI-MS/MS method for the determination of andrographolide in human plasma

A liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS) method for the determination of andrographolide in human plasma was established. Dehydroandrographolide was used as the internal standard (I.S.). The plasma samples were deproteinized with methanol and separate...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-02, Vol.877 (5), p.502-506
Main Authors: Xu, Li, Xiao, Da-wei, Lou, Sheng, Zou, Jian-jun, Zhu, Yu-bing, Fan, Hong-wei, Wang, Guang-ji
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Andrographolide
Anti-Inflammatory Agents - administration & dosage
Anti-Inflammatory Agents - blood
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - pharmacokinetics
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid - methods
Determination
Diterpenes - administration & dosage
Diterpenes - blood
Diterpenes - chemistry
Diterpenes - pharmacokinetics
Drug Stability
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC–ESI-MS/MS
Humans
Male
Medical sciences
Pharmacokinetics
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:A liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS) method for the determination of andrographolide in human plasma was established. Dehydroandrographolide was used as the internal standard (I.S.). The plasma samples were deproteinized with methanol and separated on a Hanbon C 18 column with a mobile phase of methanol–water (70:30, v/v). HPLC–ESI-MS/MS was performed in the selected ion monitoring (SIM) mode using target ions at [M−H 2O–H] −, m/ z 331.1 for andrographolide and [M−H] −, m/ z 331.1 for the I.S. Calibration curve was linear over the range of 1.0–150.0 ng/mL. The chromatographic separation was achieved in less than 6.5 min. The lower limits of quantification (LLOQ) was 1.0 ng/mL. The intra and inter-run precisions were less than 6.95 and 7.22%, respectively. The method was successfully applied to determine the plasma concentrations of andrographolide in Chinese volunteers.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.12.065