Novel reovirus isolation from an Ostrich ( Struthio camelus) in Japan

• Published in: Veterinary microbiology 2009-03, Vol.134 (3), p.227-232
• Main Authors: Sakai, Kouji, Ueno, Yuichi, Ueda, Shuhei, Yada, Kaori, Fukushi, Shuetsu, Saijo, Masayuki, Kurane, Ichiro, Mutoh, Kenichiro, Yoshioka, Kazuki, Nakamura, Masayuki, Takehara, Kazuaki, Morikawa, Shigeru, Mizutani, Tetsuya
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Avian orthoreovirus; Biological and medical sciences; bird diseases; Bird Diseases - epidemiology; Bird Diseases - virology; case studies; disease detection; electron microscopy; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Viral - physiology; genetic variation; Japan - epidemiology; Microbiology; Miscellaneous; molecular epidemiology; Molecular Sequence Data; nucleotide sequences; Orthoreovirus; Orthoreovirus, Avian - classification; Orthoreovirus, Avian - genetics; Orthoreovirus, Avian - isolation & purification; Ostrich; ostriches; pathogen identification; Phylogeny; rapid methods; RDV; Reoviridae Infections - epidemiology; Reoviridae Infections - veterinary; Reoviridae Infections - virology; Reovirus; reverse transcriptase polymerase chain reaction; RNA; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; sequence analysis; sigma A protein; sigma factors; species differences; Struthio camelus; Struthioniformes - virology; Viral Core Proteins - chemistry; Viral Core Proteins - genetics; Viral Core Proteins - metabolism; viral diseases of animals and humans; viral proteins; Virology
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2008.08.022

An orthoreovirus was isolated from an Ostrich ( Struthio camelus) and rapidly identified as orthoreovirus by the rapid determination of viral RNA sequences (RDV) system and electron microscopy. Phylogenetic analysis of the sigma A protein indicated that the isolate belonged to avian species and was closely related to chicken orthoreovirus strain 138. The results of the present study indicated that an ostrich orthoreovirus is slight different from other chicken orthoreoviruses and provided evidence of diversity among avian orthoreoviruses. To our knowledge, this is the first genetic report of an orthoreovirus isolated from an ostrich.