Overexpression of a NAC transcription factor enhances rice drought and salt tolerance

The plant-specific NAC (NAM, ATAF1/2, CUC2) transcription factors play diverse roles in plant development and stress responses. In this study, a rice NAC gene, ONAC045, was functionally characterized, especially with regard to its role in abiotic stress resistance. Expression analysis revealed that...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-02, Vol.379 (4), p.985-989
Main Authors: Zheng, Xingnan, Chen, Bo, Lu, Guojun, Han, Bin
Format: Article
Language:English
Subjects:
Abiotic stress
Allium cepa
Amino Acid Sequence
Drought
Droughts
Gene Expression Regulation, Plant
Molecular Sequence Data
Oryza - genetics
Oryza - physiology
Oryza sativa
Salt
Salt-Tolerance - genetics
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transcriptional Activation
Transcriptional activator
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Summary:The plant-specific NAC (NAM, ATAF1/2, CUC2) transcription factors play diverse roles in plant development and stress responses. In this study, a rice NAC gene, ONAC045, was functionally characterized, especially with regard to its role in abiotic stress resistance. Expression analysis revealed that ONAC045 was induced by drought, high salt, and low temperature stresses, and abscisic acid (ABA) treatment in leaves and roots. Transcriptional activation assay in yeast indicated that ONAC045 functioned as a transcriptional activator. Transient expression of GFP-ONAC045 in onion epidermal cells revealed that ONAC045 protein was localized in the nucleus. Transgenic rice plants overexpressing ONAC045 showed enhanced tolerance to drought and salt treatments. Two stress-responsive genes were upregulated in transgenic rice. Together, these results suggest that ONAC045 encodes a novel stress-responsive NAC transcription factor and is potential useful for engineering drought and salt tolerant rice.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.12.163