Novel SNP of the goat prolactin gene (PRL) associated with cashmere traits

Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits...

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Bibliographic Details
Published in:Journal of applied genetics 2009, Vol.50 (1), p.51-54
Main Authors: Lan, X-Y, Pan, Ch-Y, Chen, H, Lei, Ch-Z, Li, F-Y, Zhang, H-Y, Ni, Y-S
Format: Article
Language:English
Subjects:
Alleles
Animals
DNA Mutational Analysis
Gene Frequency
Genotype
Goats - genetics
Hair
Models, Genetic
Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Polymorphism, Single-Stranded Conformational
Prolactin - genetics
Seasons
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Summary:Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goat PRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed by Eco24I PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P=0.014).
ISSN:1234-1983
DOI:10.1007/BF03195652