Quantitative analysis of short-chain acyl-coenzymeAs in plant tissues by LC–MS–MS electrospray ionization method

Because acyl-CoAs play major roles in numerous anabolic and catabolic pathways, the quantitative determination of these metabolites in biological tissues is paramount to understanding the regulation of these metabolic processes. Here, we report a method for the analysis of a collection of short-chai...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-02, Vol.877 (5), p.482-488
Main Authors: Perera, M. Ann D.N., Choi, Suh-Yeon, Wurtele, Eve Syrkin, Nikolau, Basil J.
Format: Article
Language:English
Subjects:
Acyl Coenzyme A - analysis
Acyl Coenzyme A - chemistry
Acyl-CoA
Analysis
Analytical, structural and metabolic biochemistry
Arabidopsis - chemistry
Biological and medical sciences
Chromatography, Liquid - methods
Coenzyme A
Fundamental and applied biological sciences. Psychology
General pharmacology
LC–MS–MS
Medical sciences
Metabolites
Organ Specificity
Pharmacology. Drug treatments
Plant Leaves - chemistry
Plants
Reference Standards
Seedlings - chemistry
Seeds - chemistry
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:Because acyl-CoAs play major roles in numerous anabolic and catabolic pathways, the quantitative determination of these metabolites in biological tissues is paramount to understanding the regulation of these metabolic processes. Here, we report a method for the analysis of a collection of short-chain acyl-CoAs (
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.12.053