Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) prese...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-02, Vol.379 (4), p.817-823
Main Authors: Ohata, Shinya, Nawa, Makiko, Kasama, Takeshi, Yamasaki, Tokiwa, Sawanobori, Kenji, Hata, Shoji, Nakamura, Takashi, Asaoka, Yoichi, Watanabe, Toshio, Okamoto, Hitoshi, Hara, Takahiko, Terai, Shuji, Sakaida, Isao, Katada, Toshiaki, Nishina, Hiroshi
Format: Article
Language:English
Subjects:
AGM
AML1
Animals
CD44
Erythrocyte
Hematopoiesis
Hepatoblast
Hepatocytes - metabolism
Hepatogenesis
Hyaluronan Receptors - biosynthesis
Liv8
Liver
Liver - cytology
Liver - embryology
Liver - metabolism
Liver development
Mice
Mice, Inbred C57BL
Monoclonal antibody
Stem Cells - metabolism
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Summary:The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1 − / − murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.12.149