Regulation of chemokine gene expression and secretion in Staphylococcus aureus-infected osteoblasts

Staphylococcus aureus is the major cause of osteomyelitis or bone infection, leading to major morbidity, often in children. Little is known about immunopathogenesis of osteomyelitis, although uncontrolled inflammation is a major clinical feature. This study investigated effects of dexamethasone, PGE...

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Bibliographic Details
Published in:Microbes and infection 2004-07, Vol.6 (9), p.844-852
Main Authors: Wright, Kathleen M., Friedland, Jon S.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Cell Line
Chemokine CCL2 - genetics
Chemokine CCL2 - metabolism
Chemokine CCL2 - secretion
Chemokine CCL5
Chemokine CXCL10
Chemokines
Chemokines, CC - genetics
Chemokines, CC - metabolism
Chemokines, CC - secretion
Chemokines, CXC - genetics
Chemokines, CXC - metabolism
Chemokines, CXC - secretion
Cytokines - metabolism
Dexamethasone
Dexamethasone - pharmacology
Dinoprostone - pharmacology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Humans
Intercellular Signaling Peptides and Proteins - genetics
Intercellular Signaling Peptides and Proteins - metabolism
Intercellular Signaling Peptides and Proteins - secretion
Microbiology
Osteoblasts - microbiology
Osteomyelitis
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Prostaglandin E2
Staphylococcus aureus
Staphylococcus aureus - pathogenicity
T h2 cytokines
Th2 Cells - immunology
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Summary:Staphylococcus aureus is the major cause of osteomyelitis or bone infection, leading to major morbidity, often in children. Little is known about immunopathogenesis of osteomyelitis, although uncontrolled inflammation is a major clinical feature. This study investigated effects of dexamethasone, PGE 2 and T h2 cytokines, all potential down-regulatory mediators, on control of S. aureus-induced C-X-C (CXCL8, CXCL10) and C-C (CCL5, CCL2) chemokine gene expression and secretion from human osteoblastic MG-63 cells and primary NHOst cells. Chemokine mRNA expression and secretion were reduced 50–75% by dexamethasone, whereas PGE 2 doubled mRNA accumulation, as detected by RNase protection assay and RT-PCR, but decreased chemokine secretion 33–71% ( P 
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2004.04.008