Wnt/β-Catenin and Estrogen Signaling Converge in Vivo

Wnt and estrogen signaling represent important regulatory pathways, each controlling a wide range of biological processes. While an increasing number of observations suggest potential convergence between these pathways, no direct evidence of their functional interaction has been reported. Using huma...

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Bibliographic Details
Published in:The Journal of biological chemistry 2004-09, Vol.279 (39), p.40255-40258
Main Authors: Kouzmenko, Alexander P., Takeyama, Ken-ichi, Ito, Saya, Furutani, Takashi, Sawatsubashi, Shun, Maki, Akio, Suzuki, Eriko, Kawasaki, Yoshihiro, Akiyama, Tetsu, Tabata, Testuya, Kato, Shigeaki
Format: Article
Language:English
Subjects:
Animals
Apoptosis
beta Catenin
Cell Line, Tumor
Chromatin - metabolism
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Drosophila
Estrogen Receptor alpha
Estrogens - metabolism
Genes, Reporter
Humans
Immunoblotting
In Situ Nick-End Labeling
Mutation
Precipitin Tests
Promoter Regions, Genetic
Protein Binding
Receptors, Estrogen - metabolism
Signal Transduction
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription, Genetic
Transcriptional Activation
Transfection
Transgenes
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Summary:Wnt and estrogen signaling represent important regulatory pathways, each controlling a wide range of biological processes. While an increasing number of observations suggest potential convergence between these pathways, no direct evidence of their functional interaction has been reported. Using human colon and breast cancer cells, we found that estrogen receptor (ER) α- and β-catenin precipitated within the same immunocomplexes, reciprocally enhanced the transactivation of cognate reporter genes, and were reciprocally recruited to cognate response elements in the promoters of endogenous target genes. Using transgenic Drosophila that ectopically expressed human ERα alone or together with metabolically stable β-catenin/Armadillo mutants, we demonstrated genetic interaction between these signal transducers in vivo. Thus, we present here the first direct evidence of cross-talk between Wnt and estrogen signaling pathways via functional interaction between β-catenin and ERα.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.C400331200