Hot Topic: Using a Stearoyl-CoA Desaturase Transgene to Alter Milk Fatty Acid Composition

Stearoyl-CoA desaturase enzyme converts specific medium- and long-chain saturated fatty acids to their monounsaturated form. Transgenic goats expressing a bovine β-lactoglobulin promoter-rat stearoyl-CoA desaturase cDNA construct in mammary gland epithelial cells were produced by pronuclear microinj...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dairy science 2004-10, Vol.87 (10), p.3510-3514
Main Authors: Reh, W.A., Maga, E.A., Collette, N.M.B., Moyer, A., Conrad-Brink, J.S., Taylor, S.J., DePeters, E.J., Oppenheim, S., Rowe, J.D., BonDurant, R.H., Anderson, G.B., Murray, J.D.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Cattle
conjugated linoleic acid
DNA, Complementary - genetics
Epithelial Cells - enzymology
fatty acid composition
Fatty Acids - analysis
Fatty Acids, Monounsaturated - analysis
Female
Gene Expression
goat milk
goats
Goats - genetics
Lactation
Lactoglobulins - genetics
Linoleic Acids, Conjugated - analysis
Mammary Glands, Animal - enzymology
Milk - chemistry
milk fat
Promoter Regions, Genetic - genetics
Rats
stearoyl-CoA desaturase
Stearoyl-CoA Desaturase - genetics
Stearoyl-CoA Desaturase - metabolism
transgenic
transgenic animals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Stearoyl-CoA desaturase enzyme converts specific medium- and long-chain saturated fatty acids to their monounsaturated form. Transgenic goats expressing a bovine β-lactoglobulin promoter-rat stearoyl-CoA desaturase cDNA construct in mammary gland epithelial cells were produced by pronuclear microinjection. The fatty acid composition of milk from 4 female transgenic founders was analyzed on d 7, 14, and 30 of their first lactation. In 2 animals, the expression of the transgene changed the overall fatty acid composition of the resulting milk fat to a less saturated and more monounsaturated fatty acid profile at d 7 of lactation; however, this effect diminished by d 30. In addition, one animal had an increased proportion of the rumen-derived monounsaturated fatty acid C18:1 trans11 converted by stearoyl-CoA desaturase to the conjugated linoleic acid isomer C18:2 cis9 trans11. Milk that has higher proportions of monounsaturated fatty acids and conjugated linoleic acid may have benefits for human cardiovascular health.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(04)73486-4