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Posttranslational Modification of Brain Tubulins from the Antarctic Fish Notothenia coriiceps: Reduced C-Terminal Glutamylation Correlates with Efficient Microtubule Assembly at Low Temperature
We have shown previously that the tubulins of Antarctic fish assemble into microtubules efficiently at low temperatures (−2 to +2 °C) due to adaptations intrinsic to the tubulin subunits. To determine whether changes in posttranslational glutamylation of the fish tubulins may contribute to cold adap...
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Published in: | Biochemistry (Easton) 2004-09, Vol.43 (38), p.12265-12274 |
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description | We have shown previously that the tubulins of Antarctic fish assemble into microtubules efficiently at low temperatures (−2 to +2 °C) due to adaptations intrinsic to the tubulin subunits. To determine whether changes in posttranslational glutamylation of the fish tubulins may contribute to cold adaptation of microtubule assembly, we have characterized C-terminal peptides from α- and β-tubulin chains from brains of adult specimens of the Antarctic rockcod Notothenia coriiceps by MALDI-TOF mass spectrometry and by Edman degradation amino acid sequencing. Of the four fish β-tubulin isotypes, nonglutamylated isoforms were more abundant than glutamylated isoforms. In addition, maximal glutamyl side-chain length was shorter than that observed for mammalian brain β tubulins. For the nine fish α-tubulin isotypes, nonglutamylated isoforms were also generally more abundant than glutamylated isoforms. When glutamylated, however, the maximal side-chain lengths of the fish α tubulins were generally longer than those of adult rat brain α chains. Thus, Antarctic fish adult brain tubulins are glutamylated differently than mammalian brain tubulins, resulting in a more heterogeneous population of α isoforms and a reduction in the number of β isoforms. By contrast, neonatal rat brain tubulin possesses low levels of glutamylation that are similar to that of the adult fish brain tubulins. We suggest that unique residue substitutions in the primary structures of Antarctic fish tubulin isotypes and quantitative changes in isoform glutamylation act synergistically to adapt microtubule assembly to low temperatures. |
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For the nine fish α-tubulin isotypes, nonglutamylated isoforms were also generally more abundant than glutamylated isoforms. When glutamylated, however, the maximal side-chain lengths of the fish α tubulins were generally longer than those of adult rat brain α chains. Thus, Antarctic fish adult brain tubulins are glutamylated differently than mammalian brain tubulins, resulting in a more heterogeneous population of α isoforms and a reduction in the number of β isoforms. By contrast, neonatal rat brain tubulin possesses low levels of glutamylation that are similar to that of the adult fish brain tubulins. We suggest that unique residue substitutions in the primary structures of Antarctic fish tubulin isotypes and quantitative changes in isoform glutamylation act synergistically to adapt microtubule assembly to low temperatures.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi049070z</identifier><identifier>PMID: 15379565</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aging - physiology ; Amino Acid Sequence ; Animals ; Animals, Newborn ; Antarctic Regions ; Brain - metabolism ; Chromatography, High Pressure Liquid ; Cold Temperature ; DNA, Complementary - genetics ; Glutamine - metabolism ; Microtubules - chemistry ; Microtubules - metabolism ; Molecular Sequence Data ; Notothenia coriiceps ; Perciformes - genetics ; Protein Isoforms - chemistry ; Protein Isoforms - metabolism ; Protein Processing, Post-Translational ; Proteomics ; Rats ; Sequence Alignment ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tubulin - chemistry ; Tubulin - metabolism</subject><ispartof>Biochemistry (Easton), 2004-09, Vol.43 (38), p.12265-12274</ispartof><rights>Copyright © 2004 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a295t-135c2b8ccdeed30164994d55fe998486e04cbf59933c00c669bb96cfb84aa4ee3</citedby><cites>FETCH-LOGICAL-a295t-135c2b8ccdeed30164994d55fe998486e04cbf59933c00c669bb96cfb84aa4ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15379565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Redeker, Virginie</creatorcontrib><creatorcontrib>Frankfurter, Anthony</creatorcontrib><creatorcontrib>Parker, Sandra K</creatorcontrib><creatorcontrib>Rossier, Jean</creatorcontrib><creatorcontrib>Detrich, H. William</creatorcontrib><title>Posttranslational Modification of Brain Tubulins from the Antarctic Fish Notothenia coriiceps: Reduced C-Terminal Glutamylation Correlates with Efficient Microtubule Assembly at Low Temperature</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>We have shown previously that the tubulins of Antarctic fish assemble into microtubules efficiently at low temperatures (−2 to +2 °C) due to adaptations intrinsic to the tubulin subunits. To determine whether changes in posttranslational glutamylation of the fish tubulins may contribute to cold adaptation of microtubule assembly, we have characterized C-terminal peptides from α- and β-tubulin chains from brains of adult specimens of the Antarctic rockcod Notothenia coriiceps by MALDI-TOF mass spectrometry and by Edman degradation amino acid sequencing. Of the four fish β-tubulin isotypes, nonglutamylated isoforms were more abundant than glutamylated isoforms. In addition, maximal glutamyl side-chain length was shorter than that observed for mammalian brain β tubulins. For the nine fish α-tubulin isotypes, nonglutamylated isoforms were also generally more abundant than glutamylated isoforms. When glutamylated, however, the maximal side-chain lengths of the fish α tubulins were generally longer than those of adult rat brain α chains. Thus, Antarctic fish adult brain tubulins are glutamylated differently than mammalian brain tubulins, resulting in a more heterogeneous population of α isoforms and a reduction in the number of β isoforms. By contrast, neonatal rat brain tubulin possesses low levels of glutamylation that are similar to that of the adult fish brain tubulins. We suggest that unique residue substitutions in the primary structures of Antarctic fish tubulin isotypes and quantitative changes in isoform glutamylation act synergistically to adapt microtubule assembly to low temperatures.</description><subject>Aging - physiology</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antarctic Regions</subject><subject>Brain - metabolism</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cold Temperature</subject><subject>DNA, Complementary - genetics</subject><subject>Glutamine - metabolism</subject><subject>Microtubules - chemistry</subject><subject>Microtubules - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Notothenia coriiceps</subject><subject>Perciformes - genetics</subject><subject>Protein Isoforms - chemistry</subject><subject>Protein Isoforms - metabolism</subject><subject>Protein Processing, Post-Translational</subject><subject>Proteomics</subject><subject>Rats</subject><subject>Sequence Alignment</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Tubulin - chemistry</subject><subject>Tubulin - metabolism</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi0EotvCgRdAvoDEIcVOYifmVlbdttIuVDT0ajnOROuSxIvtqF1OXPtefRKeBC9ZlQsSvthjf5pP4x-hV5QcU5LS97UhuSAF-fEEzShLSZILwZ6iGSGEJ6ng5AAden8Ty5wU-XN0QFlWCMbZDD1cWh-CU4PvVDB2UB1e2ca0Rv8psW3xR6fMgKuxHjszeNw62-OwBnwyBOV0MBovjF_jTzbYeD0YhbV1xmjY-A-_ft7jL9CMGho8TypwvdkpzroxqH47KfHcOgfxDB7fmrDGp23UGxgCXhntbNiZo8576Otui1XAS3uLK-g34FQYHbxAz1rVeXi534_Q18VpNT9Plp_PLuYny0SlgoWEZkyndal1A9BkhPL4TXnDWAtClHnJgeS6bpkQWaYJ0ZyLuhZct3WZK5UDZEfo7dR34-z3EXyQvfEauk4NYEcvOS9FKgrxX5AWGRVxRfDdBMY5vXfQyo0zvXJbSYncZSsfs43s633Tse6h-Uvuw4xAMgHGB7h7fFfum-RFVjBZXV7J1TU9v14UXF5F_s3EK-3ljR1djMb_Q_wb5BTAhQ</recordid><startdate>20040928</startdate><enddate>20040928</enddate><creator>Redeker, Virginie</creator><creator>Frankfurter, Anthony</creator><creator>Parker, Sandra K</creator><creator>Rossier, Jean</creator><creator>Detrich, H. William</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20040928</creationdate><title>Posttranslational Modification of Brain Tubulins from the Antarctic Fish Notothenia coriiceps: Reduced C-Terminal Glutamylation Correlates with Efficient Microtubule Assembly at Low Temperature</title><author>Redeker, Virginie ; Frankfurter, Anthony ; Parker, Sandra K ; Rossier, Jean ; Detrich, H. William</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a295t-135c2b8ccdeed30164994d55fe998486e04cbf59933c00c669bb96cfb84aa4ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aging - physiology</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antarctic Regions</topic><topic>Brain - metabolism</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cold Temperature</topic><topic>DNA, Complementary - genetics</topic><topic>Glutamine - metabolism</topic><topic>Microtubules - chemistry</topic><topic>Microtubules - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Notothenia coriiceps</topic><topic>Perciformes - genetics</topic><topic>Protein Isoforms - chemistry</topic><topic>Protein Isoforms - metabolism</topic><topic>Protein Processing, Post-Translational</topic><topic>Proteomics</topic><topic>Rats</topic><topic>Sequence Alignment</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Tubulin - chemistry</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redeker, Virginie</creatorcontrib><creatorcontrib>Frankfurter, Anthony</creatorcontrib><creatorcontrib>Parker, Sandra K</creatorcontrib><creatorcontrib>Rossier, Jean</creatorcontrib><creatorcontrib>Detrich, H. William</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redeker, Virginie</au><au>Frankfurter, Anthony</au><au>Parker, Sandra K</au><au>Rossier, Jean</au><au>Detrich, H. William</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Posttranslational Modification of Brain Tubulins from the Antarctic Fish Notothenia coriiceps: Reduced C-Terminal Glutamylation Correlates with Efficient Microtubule Assembly at Low Temperature</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>2004-09-28</date><risdate>2004</risdate><volume>43</volume><issue>38</issue><spage>12265</spage><epage>12274</epage><pages>12265-12274</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>We have shown previously that the tubulins of Antarctic fish assemble into microtubules efficiently at low temperatures (−2 to +2 °C) due to adaptations intrinsic to the tubulin subunits. To determine whether changes in posttranslational glutamylation of the fish tubulins may contribute to cold adaptation of microtubule assembly, we have characterized C-terminal peptides from α- and β-tubulin chains from brains of adult specimens of the Antarctic rockcod Notothenia coriiceps by MALDI-TOF mass spectrometry and by Edman degradation amino acid sequencing. Of the four fish β-tubulin isotypes, nonglutamylated isoforms were more abundant than glutamylated isoforms. In addition, maximal glutamyl side-chain length was shorter than that observed for mammalian brain β tubulins. For the nine fish α-tubulin isotypes, nonglutamylated isoforms were also generally more abundant than glutamylated isoforms. When glutamylated, however, the maximal side-chain lengths of the fish α tubulins were generally longer than those of adult rat brain α chains. Thus, Antarctic fish adult brain tubulins are glutamylated differently than mammalian brain tubulins, resulting in a more heterogeneous population of α isoforms and a reduction in the number of β isoforms. By contrast, neonatal rat brain tubulin possesses low levels of glutamylation that are similar to that of the adult fish brain tubulins. We suggest that unique residue substitutions in the primary structures of Antarctic fish tubulin isotypes and quantitative changes in isoform glutamylation act synergistically to adapt microtubule assembly to low temperatures.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>15379565</pmid><doi>10.1021/bi049070z</doi><tpages>10</tpages></addata></record> |
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subjects | Aging - physiology Amino Acid Sequence Animals Animals, Newborn Antarctic Regions Brain - metabolism Chromatography, High Pressure Liquid Cold Temperature DNA, Complementary - genetics Glutamine - metabolism Microtubules - chemistry Microtubules - metabolism Molecular Sequence Data Notothenia coriiceps Perciformes - genetics Protein Isoforms - chemistry Protein Isoforms - metabolism Protein Processing, Post-Translational Proteomics Rats Sequence Alignment Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tubulin - chemistry Tubulin - metabolism |
title | Posttranslational Modification of Brain Tubulins from the Antarctic Fish Notothenia coriiceps: Reduced C-Terminal Glutamylation Correlates with Efficient Microtubule Assembly at Low Temperature |
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