new preparation method to study fresh plant structures with X-ray computed tomography

Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we pr...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2009, Vol.233 (1), p.1-4
Main Authors: LEROUX, O, LEROUX, F, BELLEFROID, E, CLAEYS, M, COUVREUR, M, BORGONIE, G, VAN HOOREBEKE, L, MASSCHAELE, B, VIANE, R
Format: Article
Language:English
Subjects:
3-D
Computed tomography
critical point drying
Desiccation
en bloc staining
fern root
Plant Structures - ultrastructure
sample preparation
Specimen Handling - methods
Staining and Labeling - methods
Tomography, X-Ray Computed
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Summary:Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2008.03088.x