Role of cyclooxygenase-1 and -2 in satellite cell proliferation, differentiation, and fusion

Skeletal muscle satellite cells play an important role in muscle regeneration. Previous work has suggested that nonsteroidal anti‐inflammatory drugs may inhibit their activity. We cultured skeletal muscle satellite cells from 9‐month‐old Sprague‐Dawley rats and exposed them to naproxen sodium (a non...

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Bibliographic Details
Published in:Muscle & nerve 2004-10, Vol.30 (4), p.497-500
Main Authors: Mendias, Christopher L., Tatsumi, Ryuichi, Allen, Ronald E.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Cell Differentiation - physiology
Cell Division - physiology
Cell Fusion
Cells, Cultured
Culture Media
cyclooxygenase
Cyclooxygenase 1
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Isoenzymes - physiology
Luminescent Measurements
Male
Membrane Proteins
muscle regeneration
Myogenin - biosynthesis
Naproxen
Nitrobenzenes
NSAID
prostaglandin
Prostaglandin-Endoperoxide Synthases - physiology
Pyrazoles
Rats
Rats, Sprague-Dawley
satellite cell
Satellite Cells, Skeletal Muscle - enzymology
Satellite Cells, Skeletal Muscle - physiology
Striated muscle. Tendons
Sulfonamides
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:Skeletal muscle satellite cells play an important role in muscle regeneration. Previous work has suggested that nonsteroidal anti‐inflammatory drugs may inhibit their activity. We cultured skeletal muscle satellite cells from 9‐month‐old Sprague‐Dawley rats and exposed them to naproxen sodium (a nonselective cyclooxygenase inhibitor), NS‐398 (a selective cyclooxygenase‐2 inhibitor), and SC‐560 (a selective cyclooxygenase‐1 inhibitor) for 96 h. Cyclooxygenase‐2 inhibition alone resulted in decreased satellite cell proliferation, and inhibition of both cyclooxygenase‐1 and cyclooxygenase‐2 resulted in decreased satellite cell differentiation and fusion. This study suggests that the cyclooxygenase enzymes appear to play an important part in satellite cell proliferation, differentiation, and fusion and that nonsteroidal anti‐inflammatory medication may have an adverse effect on muscle regeneration following injury. The use of a selective cyclooxygenase‐2 inhibitor over nonspecific cyclooxygenase inhibitors in the treatment of muscle injuries is not supported. Muscle Nerve 30: 497–500, 2004
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.20102