A solid-phase blocking ELISA for detection of antibodies to Nipah virus

A monoclonal antibody (MAb) based solid-phase blocking ELISA was developed for detection of antibodies to Nipah virus. The ELISA was designed to detect remaining antigens on the plate with anti-Nipah MAb conjugate after the reaction with sample serum, and enabled simple procedure, detection of neutr...

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Bibliographic Details
Published in:Journal of virological methods 2004-11, Vol.121 (2), p.259-261
Main Authors: Kashiwazaki, Yoshihito, Na, Yeoh No, Tanimura, Nobuhiko, Imada, Tadao
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibodies, Viral - blood
Antigens, Viral
Bats
Biological and medical sciences
Blocking ELISA
Cattle
Chiroptera - blood
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Fundamental and applied biological sciences. Psychology
Henipavirus Infections - blood
Henipavirus Infections - epidemiology
Henipavirus Infections - veterinary
Humans
Malaysia
Malaysia - epidemiology
Mass Screening - methods
Microbiology
Neutralization Tests - standards
Nipah virus
Nipah Virus - immunology
Nipah Virus - isolation & purification
Pteropodidae
Swine - blood
Techniques used in virology
Virology
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Summary:A monoclonal antibody (MAb) based solid-phase blocking ELISA was developed for detection of antibodies to Nipah virus. The ELISA was designed to detect remaining antigens on the plate with anti-Nipah MAb conjugate after the reaction with sample serum, and enabled simple procedure, detection of neutralizing antibody to Nipah virus, and application of samples from different animal species. Forty of 200 swine reference sera examined were positive by the ELISA, of which thirty seven were found positive by serum neutralization test. Sera from a total of 131 fruit bats captured in Malaysia were also tested and all found negative by the both tests. It is considered that the solid-phase blocking ELISA can be used as a screening test for Nipah virus infection followed by the serum neutralization test as confirmatory test.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2004.06.015