A solid-phase blocking ELISA for detection of antibodies to Nipah virus

• Published in: Journal of virological methods 2004-11, Vol.121 (2), p.259-261
• Main Authors: Kashiwazaki, Yoshihito, Na, Yeoh No, Tanimura, Nobuhiko, Imada, Tadao
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Antibodies, Viral - blood; Antigens, Viral; Bats; Biological and medical sciences; Blocking ELISA; Cattle; Chiroptera - blood; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Fundamental and applied biological sciences. Psychology; Henipavirus Infections - blood; Henipavirus Infections - epidemiology; Henipavirus Infections - veterinary; Humans; Malaysia; Malaysia - epidemiology; Mass Screening - methods; Microbiology; Neutralization Tests - standards; Nipah virus; Nipah Virus - immunology; Nipah Virus - isolation & purification; Pteropodidae; Swine - blood; Techniques used in virology; Virology
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2004.06.015

A monoclonal antibody (MAb) based solid-phase blocking ELISA was developed for detection of antibodies to Nipah virus. The ELISA was designed to detect remaining antigens on the plate with anti-Nipah MAb conjugate after the reaction with sample serum, and enabled simple procedure, detection of neutralizing antibody to Nipah virus, and application of samples from different animal species. Forty of 200 swine reference sera examined were positive by the ELISA, of which thirty seven were found positive by serum neutralization test. Sera from a total of 131 fruit bats captured in Malaysia were also tested and all found negative by the both tests. It is considered that the solid-phase blocking ELISA can be used as a screening test for Nipah virus infection followed by the serum neutralization test as confirmatory test.