Glycosphingolipid composition of primary cultured human brain microvascular endothelial cells

Glycosphingolipid (GSL) antigens have been considered to be involved in the pathogenesis of autoimmune neurologic disorders including multiple sclerosis. To establish the GSL pattern specific for endothelial cells forming blood–brain barrier (BBB), we established a method to yield sufficient quantit...

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Published in:Journal of neuroscience research 2004-10, Vol.78 (1), p.141-150
Main Authors: Kanda, Takashi, Ariga, Toshio, Kubodera, Hisako, Jin, Hong Lian, Owada, Kiyoshi, Kasama, Takeshi, Yamawaki, Masanaga, Mizusawa, Hidehiro
Format: Article
Language:English
Subjects:
Aged
blood-brain barrier
Blood-Brain Barrier - chemistry
Brain - blood supply
Brain Chemistry
Cells, Cultured
Endothelium, Vascular - chemistry
glycosphingolipid
Glycosphingolipids - chemistry
Glycosphingolipids - isolation & purification
human brain microvascular endothelial cell
Humans
Male
Microcirculation - chemistry
sulfoglucuronosyl paragloboside
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Summary:Glycosphingolipid (GSL) antigens have been considered to be involved in the pathogenesis of autoimmune neurologic disorders including multiple sclerosis. To establish the GSL pattern specific for endothelial cells forming blood–brain barrier (BBB), we established a method to yield sufficient quantities of highly purified human brain microvascular endothelial cells (HBMECs) and compared their GSL composition to that of human umbilical cord vein endothelial cells (HUVECs), as the representative of endothelial cells not forming BBB. The major gangliosides were GM3 and sialyl paragloboside (LM1), and the major neutral GSLs were lactosylceramide (LacCer), globotriaosylceramide (Gb3), and globoside (Gb4). Trace amounts of GM1, GD1a, GD1b, GT1b, and sulfoglucuronosyl paragloboside (SGPG) could be detected by the high performance thin layer chromatography‐overlay method. SGPG was detected only at a nonconfluent state in an amount almost 1/30 that of in nonconfluent HUVECs. Conversely, GM3 and LM1 increased significantly after confluency. The amount of Gb3 in HBMECs was almost as twice that in HUVECs. The significance of these differences in GSL content between HBMECs and HUVECs and between confluent and nonconfluent states is obscure. It might be related, however, to the defense mechanism at the BBB and to the susceptibility of the central nervous system in some disorders that target cell surface GSL, such as hemolytic uremic syndrome. © 2004 Wiley‐Liss, Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/jnr.20228