Iridoids from the aerial parts of Verbena littoralis (Verbenaceae)

• Published in: Phytochemistry (Oxford) 2004-08, Vol.65 (16), p.2369-2372
• Main Authors: Castro-Gamboa, Ian, Castro, Oscar
• Format: Article
• Language: English
• Subjects: Animals; Antibacterial activity; antibacterial properties; Antioxidant; antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bacillus subtilis; Biological and medical sciences; Chemical constitution; chemical structure; chemotaxonomy; Chromatography, High Pressure Liquid; free radical scavenging activity; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; intestinal peristaltic reflex; intestines; Intestines - metabolism; Iridoids; Iridoids - chemistry; Iridoids - isolation & purification; Iridoids - pharmacology; Klebsiella pneumoniae; medicinal plants; Mice; Oxidation-Reduction; Peristalsis - drug effects; phytochemicals; Plant cytology, morphology, systematics, chorology and evolution; plant extracts; Plant physiology and development; rats; spectral analysis; Spermatophyta; stereochemistry; Stereoisomerism; Systematics (diagnosis, chromosome numbers); Verbena; Verbena littoralis; Verbenaceae; Verbenaceae - chemistry
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2004.07.008

Iridoids ( 1) and ( 2), were isolated from the aerial parts of Verbena littoralis. Both showed moderate in vitro activity against gram positive and negative bacteria, and peristaltic action in the mouse. Free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) as well as antioxidant activity as evidenced by redox properties measured on ElCD-HPLCl was observed. The iridoids, 6 S-hydroxy-8 S-methyl-4-methylene-hexahydro-cyclopenta[ c]pyran-3-one and 6 S,9 S-dihydroxy-8S-methyl-4-methylene-hexahydro-cyclopenta[ c]pyran-3-one, were isolated from the aerial parts of Verbena littoralis. Their structures and stereochemistry were elucidated by means of NMR spectral data analysis. Both compounds showed moderate in vitro activity against gram positive and negative bacteria as well as moderate in vivo intestinal peristaltic action in mouse. The iridoids also showed moderate free radical scavenging activity against l,l-diphenyl-2-picrylhydrazyl (DPPH) as well as antioxidant activity, the latter being evidenced by redox properties measured using E1CD-HPLC.