Inhibition of Centrosome Separation after DNA Damage: A Role for Nek2

• Published in: Radiation research 2004-08, Vol.162 (2), p.128-135
• Main Authors: Fletcher, Lynda, Cerniglia, George J., Nigg, Erich A., Yen, Tim J., Muschel, Ruth J.
• Format: Article
• Language: English
• Subjects: Animal cells; Base Sequence; Blotting, Western; Cell cycle; Cell lines; Centrosome - radiation effects; Centrosomes; DNA Damage; DNA Primers; Fluorescent Antibody Technique; G2 Phase - radiation effects; Gene expression regulation; HeLa cells; Humans; Irradiation; Mitosis; NIMA-Related Kinases; Precipitin Tests; Protein-Serine-Threonine Kinases - physiology; REGULAR ARTICLES; Small interfering RNA; Space life sciences; Tumor Cells, Cultured
• ISSN: 0033-7587; 1938-5404
• DOI: 10.1667/RR3211

Fletcher, L., Cerniglia, G. J., Nigg, E. A., Yen, T. J. and Muschel, R. J. Inhibition of Centrosome Separation after DNA Damage: A Role for Nek2. Radiat. Res. 162, 128–135 (2004). DNA damage results in cell cycle arrest in G2. Centrosomes also separate in G2, raising the question of whether separation occurs during the DNA damage-induced G2 arrest. Nek2, the mammalian homologue of NIMA, is a cell cycle-regulated serine/threonine protein kinase that regulates centrosome separation during G2. Here we show that damaged cells fail to activate Nek2. Both Nek2 levels and activity are reduced after DNA damage. Radiation inhibits the premature centrosome splitting induced by overexpression of Nek2, indicating that Nek2 is involved in activation of the G2 checkpoint and is not secondary to cell cycle arrest. We confirm using siRNA that centrosome separation and cell growth are impaired in the absence of Nek2. These studies define a previously unreported DNA damage response of inhibition of centrosome separation mechanistically linked to Nek2.