Regulation of Id2 expression in EL4 T lymphoma cells overexpressing growth hormone

• Published in: Cellular immunology 2009, Vol.255 (1), p.46-54
• Main Author: Weigent, Douglas A.
• Format: Article
• Language: English
• Subjects: Animals; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Growth hormone; Growth Hormone - genetics; Growth Hormone - metabolism; Humans; Id proteins; Inhibitor of Differentiation Protein 2 - genetics; Inhibitor of Differentiation Protein 2 - metabolism; Lymphoma; Mice; Promoter Regions, Genetic; STAT Transcription Factors - genetics; STAT Transcription Factors - metabolism; T lymphoma cells
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2008.10.003

In previous studies, we have shown that overexpression of growth hormone (GH) in cells of the immune system upregulates proteins involved in cell growth and protects from apoptosis. Here, we report that overexpression of GH in EL4 T lymphoma cells (GHo) also significantly increased levels of the inhibitor of differentiation-2 (Id2). The increase in Id2 was suggested in both Id2 promoter luciferase assays and by Western analysis for Id2 protein. To identify the regulatory elements that mediate transcriptional activation by GH in the Id2 promoter, promoter deletion analysis was performed. Deletion analysis revealed that transactivation involved a 301–132 bp region upstream to the Id2 transcriptional start site. The pattern in the human GHo Jurkat T lymphoma cell line paralleled that found in the mouse GHo EL4 T lymphoma cell line. Significantly less Id2 was detected in the nucleus of GHo EL4 T lymphoma cells compared to vector alone controls. Although serum increased the levels of Id2 in control vector alone cells, no difference was found in the total levels of Id2 in GHo EL4 T lymphoma cells treated with or without serum. The increase in Id2 expression in GHo EL4 T lymphoma cells measured by Id2 promoter luciferase expression and Western blot analysis was blocked by the overexpression of a dominant-negative mutant of STAT5. The results suggest that in EL4 T lymphoma cells overexpressing GH, there is an upregulation of Id2 protein that appears to involve STAT protein activity.