Chiral separation of β-adrenergic blockers on CelluCoat column by HPLC

The chiral separation of 10 β-adrenergic blockers (acebutalol, alprenolol, bufuralol, bisoprolol, celiprolol, carazolol, indenolol, metoprolol, oxprenolol and propranolol) was achieved on CelluCoat column (250 mm × 4.6 mm, 5 μm particle size). The mobile phases used were (90:10:0.2, v/v/v) and (95:5...

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Bibliographic Details
Published in:Talanta (Oxford) 2009-04, Vol.78 (2), p.458-463
Main Authors: Ali, Imran, Gaitonde, Vinay D., Aboul-Enein, Hassan Y., Hussain, Afzal
Format: Article
Language:English
Subjects:
Adrenergic beta-Antagonists - analysis
Adrenergic beta-Antagonists - isolation & purification
Analytical chemistry
CelluCoat column
Chemistry
Chiral separation
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Electrons
Exact sciences and technology
Hydrogen Bonding
Other chromatographic methods
Solvents
Stereoisomerism
Supra-molecular mechanism
β-adrenergic blockers
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Summary:The chiral separation of 10 β-adrenergic blockers (acebutalol, alprenolol, bufuralol, bisoprolol, celiprolol, carazolol, indenolol, metoprolol, oxprenolol and propranolol) was achieved on CelluCoat column (250 mm × 4.6 mm, 5 μm particle size). The mobile phases used were (90:10:0.2, v/v/v) and (95:5:0.2, v/v/v) combinations of n-heptane–ethanol–diethylamine, respectively. The flow rates were 0.5, 1.0 and 2.0 mL min −1 with detection at 225 nm. The capacity ( k), selectivity ( α) and resolution ( R s) factors were 0.44–12.91, 1.12–2.19 and 1.00–9.50, respectively. The proposed supra-molecular models indicated that the chiral resolution were governed by π–π interactions, hydrogen bondings and steric effect.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2008.11.043