Carbon use, nitrogen use, and isotopic fractionation of ectomycorrhizal and saprotrophic fungi in natural abundance and 13C-labelled cultures

Stable isotopes in fruit bodies from field studies have been used to infer ectomycorrhizal or saprotrophic status and to understand carbon and nitrogen use, but few controlled culture studies have correlated source and fungal isotopic patterns. Here, we measured natural abundances of 15N and 13C in...

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Bibliographic Details
Published in:Mycological research 2004-07, Vol.108 (7), p.725-736
Main Authors: HOBBIE, Erik A., SÁNCHEZ, Fernando S., RYGIEWICZ, Paul T.
Format: Article
Language:English
Subjects:
Agaricus albolutescens
Agaricus subrutilescens
Amanita gemmata
Amanita muscaria
carbon
Carbon - metabolism
Carbon Isotopes
Clitocybe nebularis
culture media
ectomycorrhizae
Fungi - growth & development
Fungi - metabolism
glucose
Glucose - metabolism
isotope labeling
Lactarius deliciosus
Leucopaxillus gentianeus
Lycoperdon perlatum
Mycorrhizae - growth & development
Mycorrhizae - metabolism
mycorrhizal fungi
nitrogen
Nitrogen - metabolism
Nitrogen Isotopes
Panellus serotinus
Paxillus involutus
Pleurotus
Rhizopogon occidentalis
Russula adusta
Russula sororia
Species Specificity
stable isotopes
Stropharia ambigua
Suillus brevipes
Suillus fuscotomentosus
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Summary:Stable isotopes in fruit bodies from field studies have been used to infer ectomycorrhizal or saprotrophic status and to understand carbon and nitrogen use, but few controlled culture studies have correlated source and fungal isotopic patterns. Here, we measured natural abundances of 15N and 13C in ten strains of ectomycorrhizal fungi and seven strains of saprotrophic fungi grown on agar with three different primary carbon sources: glucose, glucose plus malt extract, and potato dextrose agar. Eight fungal strains were also grown using position-specific, 13C-labelled glucose (C-1 through C-6 labelled). Most fungi resembled nitrogen sources in δ15N, suggesting that growth on agar media minimizes isotopic fractionation on uptake compared to growth on liquid media, and that in general saprotrophic and mycorrhizal fungi process nitrogen similarly. Saprotrophic fungi were more depleted in 13C than ectomycorrhizal fungi on all media, presumably because of assimilation of 13C-depleted, agar-derived carbon. Results on 13C-enriched glucose indicated that saprotrophic fungi obtained up to 45% of their carbon from the agar substrate. Fungi generally incorporated the individual carbon atoms of glucose in the order, C-4
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756204000590