Effect of chymase-dependent transforming growth factor beta on peritoneal adhesion formation in a rat model

To clarify the role of chymase produced from mast cells, which are closely related to adhesion formation, we investigated the preventive effect of a chymase inhibitor on adhesion formation in a rat model. A lesion was created in rats by uterus scraping, and a chymase inhibitor, Suc-Val-Pro-Phep(OPh)...

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Bibliographic Details
Published in:Surgery today (Tokyo, Japan) Japan), 2004-10, Vol.34 (10), p.865-867
Main Authors: Okamoto, Yukiko, Takai, Shinji, Miyazaki, Mizuo
Format: Article
Language:English
Subjects:
Animals
Ascitic Fluid - chemistry
Chymases
Female
Oligopeptides - pharmacology
Rats
Rats, Sprague-Dawley
Serine Endopeptidases - physiology
Tissue Adhesions - physiopathology
Transforming Growth Factor beta - physiology
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Summary:To clarify the role of chymase produced from mast cells, which are closely related to adhesion formation, we investigated the preventive effect of a chymase inhibitor on adhesion formation in a rat model. A lesion was created in rats by uterus scraping, and a chymase inhibitor, Suc-Val-Pro-Phep(OPh)2 (10 microM), or a placebo was injected into the abdomen. The level of transforming growth factor beta (TGF-beta) in the peritoneal fluid was also measured. By 2 weeks after the operation, the scores for adhesion formation in the chymase inhibitor-treated group were significantly lower than those in the placebo-treated group, at 1.64 +/- 0.34 and 3.27 +/- 0.19, respectively (P < 0.01). After scraping the uterus, the level of TGF-beta in the peritoneal fluid was significantly higher in the placebo-treated group, whereas it was significantly suppressed by the chymase inhibitor. Chymase may play an important role in adhesion formation aided by TGF-beta.
ISSN:0941-1291
DOI:10.1007/s00595-004-2836-z