Quantitative Urinary Proteome Analysis for Biomarker Evaluation in Chronic Kidney Disease

A limitation of proteomic methods with respect to their clinical applicability is the lack of possibilities to directly deduce the amount of a protein or peptide from a particular mass spectrometry (MS) spectrum. For quantification of chronic kidney disease (CKD)-specific urinary polypeptides in cap...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteome research 2009-01, Vol.8 (1), p.268-281
Main Authors: Jantos-Siwy, Justyna, Schiffer, Eric, Brand, Korbinian, Schumann, Gerhard, Rossing, Kasper, Delles, Christian, Mischak, Harald, Metzger, Jochen
Format: Article
Language:English
Subjects:
Biomarkers - urine
Calibration
Electrophoresis, Capillary - methods
Female
Humans
Ions
Kidney Failure, Chronic - diagnosis
Kidney Failure, Chronic - urine
Male
Mass Spectrometry - methods
Middle Aged
Peptides - chemistry
Proteome - analysis
Proteomics - methods
ROC Curve
Urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A limitation of proteomic methods with respect to their clinical applicability is the lack of possibilities to directly deduce the amount of a protein or peptide from a particular mass spectrometry (MS) spectrum. For quantification of chronic kidney disease (CKD)-specific urinary polypeptides in capillary electrophoresis coupled with mass spectrometry (CE-MS), we compared signal intensity calibration methods based on either urinary creatinine or stable isotope labeled synthetic marker analogues (absolute quantification) with those based on ion counting using highly abundant collagen fragments as nonmarker references (relative quantification). Our results indicate that relative quantification of biomarker excretion based on ion counts in reference to endogenous “housekeeping” peptides is sufficient for the determination of urinary polypeptide levels. The calculation of absolute concentrations via exogenous stable isotope-labeled peptide standards is of no additional benefit.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr800401m