Expression of MMPs and TIMPs Family in Human ACL and MCL Fibroblasts

The human ACL (anterior cruciate ligament) is susceptible to injury but has poor healing response, whereas an injured MCL (medial collateral ligament) can be repaired relatively well. Since MMPs (matrix metalloproteases) and TIMPs (tissue inhibitor of metalloproteases) are involved in this tissue re...

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Bibliographic Details
Published in:Connective Tissue Research 2009-01, Vol.50 (1), p.7-13
Main Authors: Zhang, Jin, Yang, Li, Tang, Zhenyu, Xue, Ruyue, Wang, Yequan, Luo, Ziwei, Huang, Wei, Sung, K. L. Paul
Format: Article
Language:English
Subjects:
ACL
Anterior Cruciate Ligament - cytology
Cells, Cultured
DNA Primers - genetics
Fibroblasts - metabolism
Humans
Matrix Metalloproteinases - metabolism
MCL
Medial Collateral Ligament, Knee - cytology
MMPs
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
RT-PCR
TIMPs
Tissue Inhibitor of Metalloproteinases - metabolism
Wound Healing - physiology
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Summary:The human ACL (anterior cruciate ligament) is susceptible to injury but has poor healing response, whereas an injured MCL (medial collateral ligament) can be repaired relatively well. Since MMPs (matrix metalloproteases) and TIMPs (tissue inhibitor of metalloproteases) are involved in this tissue remodeling process, investigation of different response of MMPs and TIMPs family in ACL and MCL fibroblasts might lead to understanding the differential matrix remodeling process as well as their different healing ability. The first step would be determination of whether these tissue remodeling effectors are present in ligaments. In this study, we designed primers for real-time RT-PCR and determined the expression of MMPs and TIMPs family in ACL and MCL fibroblasts with synovium as a positive control. Semiquantitative RT-PCR revealed that multiple MMPs and TIMPs expressed in human ACL and MCL fibroblasts except MMP-8, 10, 12, 13, 15, 16, 20, and 26. MMP-7 was present in MCL but not in ACL fibroblast. Quantitative real-time RT-PCR showed that mRNA levels of MMP-1, 2, 14, 17, 23A, and 23B and TIMP-4 are significantly higher in MCL than in ACL fibroblasts. However, MMP-3 is higher in ACL than in MCL fibroblasts. We conclude that numerous MMPs and TIMPs family members that are differentially expressed in ACL and MCL might be involved in the differential matrix remodeling process as well as the differential healing ability of ACL and MCL.
ISSN:0300-8207
1521-0456
1607-8438
DOI:10.1080/03008200802376139