Small Volume Resuscitation in a Rat Model of Heatstroke

• Published in: The American journal of the medical sciences 2009-02, Vol.337 (2), p.79-87
• Main Authors: Liu, Chia-Chyuan, Cheng, Bor-Chih, Lin, Mao-Tsun, Lin, Hung-Jung
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cerebral ischemia; Cerebrovascular Disorders - etiology; Cerebrovascular Disorders - therapy; Coagulation; Disease Models, Animal; Fluid Therapy - methods; General aspects; Heat Stroke - complications; Heat Stroke - therapy; Heatstroke; Hydroxyethyl Starch Derivatives - administration & dosage; Hyper-HAES; Hypotension; Inflammation; Kidney Diseases - etiology; Kidney Diseases - therapy; Liver Diseases - etiology; Liver Diseases - therapy; Male; Medical sciences; Multiorgan dysfunction; Multiple Organ Failure - etiology; Multiple Organ Failure - therapy; Neurology; Rats; Rats, Sprague-Dawley; Resuscitation - methods; Saline Solution, Hypertonic - administration & dosage; Sodium Chloride - administration & dosage; Thrombophilia - etiology; Thrombophilia - therapy; Vascular diseases and vascular malformations of the nervous system
• ISSN: 0002-9629; 1538-2990
• DOI: 10.1097/MAJ.0b013e31817da198

Herein, we compared the effectiveness of different small volume resuscitation in a rat model of heatstroke. Anesthetized rats, immediately after the onset of heatstroke, were randomizedly divided into 5 groups and given the following: (a) nothing; (b) 0.9% NaCl (1-10mL/kg of body weight, i.v.); (c) hydroxyethyl starch (HAES) (6%, 1-10mL/kg of body weight, i.v.); (d) 7.2% NaCl (1-10mL/kg of body weight, i.v.); and (e) hyper-HAES (6% HAES plus 7.2% NaCl, 1-10mL/kg of body weight, i.v.). When the untreated or 0.9% NaCl (1-5mL/kg of body weight)-treated rats underwent heat stress, their survival time values were found to be 20 to 22minutes. Resuscitation with 10mL/kg of body weight of 0.9% NaCl, 6% HAES, 7.2% NaCl, or hyper-HAES, their survival time values, respectively, are 93 ± 6, 101 ± 12, 154 ± 18, or 286 ± 21. Apparently, the order of effectiveness in resuscitation of heatstroke is hyper-HAES > 7.2% NaCl > 0.9% NaCl or 6% HAES. The heatstroke-induced hypotension, cerebral ischemia and hypoxia, hypercoagulable state, activated inflammation, and hepatic and renal dysfunction can be significantly reduced by hyper-HAES. Our results suggest that hyper-HAES seems superior to 7.2% NaCl or HAES alone in resuscitation of heatstroke. The benefit of hyper-HAES during heatstroke is related to restoration of normal multiorgan function.