Antimycobacterial activity of synthetic pamamycins

• Published in: Journal of antimicrobial chemotherapy 2004-10, Vol.54 (4), p.824-827
• Main Authors: Lefèvre, P., Peirs, P., Braibant, M., Fauville-Dufaux, M., Vanhoof, R., Huygen, K., Wang, X.-M., Pogell, B., Wang, Y., Fischer, P., Metz, P., Content, J.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Biological and medical sciences; bioluminescence; luciferase; Luminescent Measurements; macrodiolides; Macrolides; Medical sciences; Microbial Sensitivity Tests; Molecular Structure; Mycobacterium bovis; Mycobacterium bovis - drug effects; Mycobacterium smegmatis; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Organic Chemicals; Pharmacology. Drug treatments; Streptomyces alboniger; Vibrio harveyi
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkh402

Objectives: Early studies have indicated that pamamycins, a group of macrodiolides first isolated from Streptomyces alboniger, have potent antimicrobial activity against Gram-positive bacteria, fungi and mycobacteria but not against Gram-negative bacteria. The recent availability of highly purified and reasonable quantities of several pamamycins through their total syntheses has rendered possible more extensive studies on their effects on mycobacteria. Methods: Bioluminescent strains of Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis, expressing the luxA and luxB genes from Vibrio harveyi were used for the comparison of the antimycobacterial activity of the two synthetic macrodiolides pamamycin-607 and pamamycin-621A and a non-naturally occurring cyclic dimer of pamamycin-607, i.e. yukomycin. Results: Pamamycin-607 was the most active of the three macrocycles and was more active against M. tuberculosis than against M. smegmatis. Twenty-five clinical isolates of M. tuberculosis were susceptible to pamamycin-607 in a narrow MIC range of 1.5–2.0 mg/L. The new assay was also validated by comparison with the BACTEC radiometric test. Conclusion: Rapid screening of a new class of macrocyclic antimycobacterials using bioluminescent mycobacteria identified pamamycin-607 as a potential antituberculous agent. The latter was active against clinical isolates of M. tuberculosis within a narrow MIC range of 1.5–2.0 mg/L irrespective of their resistance to isoniazid or rifampicin. Our findings warrant further investigations.