Absorption and scattering imaging of tissue with steady-state second-differential spectral-analysis tomography

A novel approach to reconstructing both the absorption and the scattering properties of a turbid medium simultaneously from steady-state broadband spectral measurements is presented that utilizes second-differential fitting to the water spectrum to estimate the optical path length in tissue. Theoret...

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Bibliographic Details
Published in:Optics letters 2004-09, Vol.29 (17), p.2043-2045
Main Authors: HENG XU, POGUE, Brian W, DEHGHANI, Hamid, PAULSEN, Keith D, SPRINGETT, Roger, DUNN, Jeff F
Format: Article
Language:English
Subjects:
Absorption
Algorithms
Animals
Biological and medical sciences
Brain - metabolism
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Hemoglobins - analysis
Hemoglobins - metabolism
Hypoxia, Brain - metabolism
Image analysis
Image Interpretation, Computer-Assisted - methods
Oxygen - analysis
Oxygen - metabolism
Phantoms, Imaging
Rats
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Infrared - instrumentation
Spectrophotometry, Infrared - methods
Spectrum Analysis - instrumentation
Spectrum Analysis - methods
Tissues, organs and organisms biophysics
Tomography - instrumentation
Tomography - methods
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Summary:A novel approach to reconstructing both the absorption and the scattering properties of a turbid medium simultaneously from steady-state broadband spectral measurements is presented that utilizes second-differential fitting to the water spectrum to estimate the optical path length in tissue. Theoretical and experimental evidence is provided to demonstrate the robust accuracy of the spectroscopy approach and reconstructed absorption images. The steady-state broadband CCD system has the potential to provide accurate chromophore imaging without the technological complexity of time- or frequency-domain systems.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.29.002043