Effects of amino acids on maturation, fertilization and embryo development of pig follicular oocytes in two IVM media

This study was conducted to develop a serum-free, defined medium for IVM of pig oocytes. Modified North Carolina State University (mNCSU)-23 media with or without supplementation with both epidermal growth factor (EGF) and gonadotrophin were used as base media. In separate experiments, each base med...

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Bibliographic Details
Published in:Theriogenology 2004-11, Vol.62 (8), p.1473-1482
Main Authors: Hong, J.Y., Yong, H.Y., Lee, B.C., Hwang, W.S., Lim, J.M., Lee, E.S.
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - pharmacology
Amino Acids, Essential - pharmacology
Animals
Blastocyst - physiology
Culture Media
Defined medium
embryo (animal)
embryo culture
Embryo Culture Techniques - veterinary
embryogenesis
Embryonic Development - drug effects
Epidermal Growth Factor - pharmacology
Female
Fertilization in Vitro - veterinary
Follicular Fluid
in vitro maturation
Male
Maturation
Oocytes
Oocytes - drug effects
Oocytes - growth & development
Pig
Polyvinyl Alcohol
Sperm-Ovum Interactions
swine
Swine - embryology
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Summary:This study was conducted to develop a serum-free, defined medium for IVM of pig oocytes. Modified North Carolina State University (mNCSU)-23 media with or without supplementation with both epidermal growth factor (EGF) and gonadotrophin were used as base media. In separate experiments, each base medium was supplemented with porcine follicular fluid (pFF), polyvinyl alcohol (PVA), PVA and essential amino acids (EAA), PVA and nonessential amino acids (NEAA) or PVA with both EAA and NEAA. Averaged across these five treatments, the percentage of blastocyst formation was higher ( P
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2004.02.013