Evaluation of an anti-rPA IgG ELISA for measuring the antibody response in mice

A recombinant protective antigen (rPA)-based enzyme-linked immunosorbent assay (ELISA) was developed to measure the serological response of female A/J mice after inoculation with the new rPA-based anthrax vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, ac...

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Bibliographic Details
Published in:Biologicals 2004-06, Vol.32 (2), p.62-69
Main Authors: Little, S.F., Webster, W.M., Norris, S.L.W., Andrews, G.P.
Format: Article
Language:English
Subjects:
Animals
Anthrax
Anthrax Vaccines - pharmacology
Antibodies, Monoclonal - chemistry
Antibody Formation
Binding, Competitive
Clinical Trials as Topic
Dose-Response Relationship, Drug
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Evaluation Studies as Topic
Female
Immunoglobulin G - chemistry
Linear Models
Logistic Models
Mice
Protein Binding
Recombinant vaccine
Sensitivity and Specificity
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Description
Summary:A recombinant protective antigen (rPA)-based enzyme-linked immunosorbent assay (ELISA) was developed to measure the serological response of female A/J mice after inoculation with the new rPA-based anthrax vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, accuracy, linearity, and stability. Experimental results suggested that the quantitative anti-rPA IgG ELISA could be used to measure antibody levels in female A/J mice and may be useful as a potency assay to monitor consistency of manufacture of a rPA-based vaccine for planned clinical trials.
ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2004.02.001