Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon

A Bacillus subtilis strain with a base substitution in the ribosome-binding site of spoVAC was temperature sensitive (ts) in sporulation and spores prepared at the permissive temperature were ts in l-alanine-triggered germination, but not in germination with Ca 2+-dipicolinic acid (DPA) or dodecylam...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-10, Vol.239 (1), p.71-77
Main Authors: Vepachedu, Venkata Ramana, Setlow, Peter
Format: Article
Language:English
Subjects:
Alanine
Amines
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - physiology
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Binding sites
Biological and medical sciences
Calcium
Calcium ions
Dipicolinic acid
Dodecylamine
Fundamental and applied biological sciences. Psychology
Germination
Growth, nutrition, cell differenciation
L-Alanine
Microbiology
Missense mutation
Molecular Sequence Data
Mutation
Nutrient release
Operon
Picolinic Acids
Spore germination
Spores
Spores, Bacterial - physiology
Sporulation
SpoVA proteins
Temperature
Temperature effects
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Summary:A Bacillus subtilis strain with a base substitution in the ribosome-binding site of spoVAC was temperature sensitive (ts) in sporulation and spores prepared at the permissive temperature were ts in l-alanine-triggered germination, but not in germination with Ca 2+-dipicolinic acid (DPA) or dodecylamine. Spores of a ts spo mutant with a missense mutation in the spoVAC coding region were not ts for germination with l-alanine, dodecylamine or Ca 2+-DPA. These findings are discussed in light of the proposal that SpoVA proteins are involved not only in DPA uptake during sporulation, but also in DPA release during nutrient-mediated spore germination.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.08.022