Role of thrombin in activation of neurons in rat hippocampus

The effect of thrombin, an agonist of proteinase-activated receptor (PAR) family, was studied on cultured rat hippocampal neurons. Thrombin in a concentration range of 1 pM - 10 nM induced a transitory dose-dependent increase in intracellular free calcium concentration. Involvement of PAR1 in neural...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2004-05, Vol.137 (5), p.453-456
Main Authors: Kiseleva, E V, Storozhevykh, T P, Pinelis, V G, Gluza, E, Strukova, S M
Format: Article
Language:English
Subjects:
Animals
Calcium - analysis
Calcium - metabolism
Cells, Cultured
Hippocampus - cytology
Hippocampus - metabolism
Indoles - pharmacology
Neurons - chemistry
Neurons - drug effects
Neurons - metabolism
Rats
Rats, Wistar
Thrombin - pharmacology
Thrombin - physiology
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Summary:The effect of thrombin, an agonist of proteinase-activated receptor (PAR) family, was studied on cultured rat hippocampal neurons. Thrombin in a concentration range of 1 pM - 10 nM induced a transitory dose-dependent increase in intracellular free calcium concentration. Involvement of PAR1 in neural response to thrombin was corroborated in experiments with TFLLRN, a selective synthetic peptide agonist of these receptors. In a calcium-free medium and after treatment with cyclopiazonic acid (inhibitor of Ca(2+)-ATPase in the endoplasmic reticulum) activation of PAR not only mobilized Ca(2+) from intracellular stores, but also induced Ca(2+) entry into the cells. Thrombin decreased Ca(2+) signal triggered by activation of NMDA-subtype glutamate receptors.
ISSN:0007-4888
1573-8221
DOI:10.1023/B:BEBM.0000038151.56296.45