Polyphasic taxonomic characterization of Lactobacillus rossiae isolates from Belgian and Italian sourdoughs reveals intraspecific heterogeneity

(GTG) 5-PCR fingerprinting and pheS sequence analysis of 18 Lactobacillus rossiae isolates, mainly originating from Belgian and Italian artisan sourdoughs, revealed intraspecies grouping as evidenced by the delineation of three and two subgroups, respectively. On the other hand, 16S rRNA and rpoA ge...

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Bibliographic Details
Published in:Systematic and applied microbiology 2009-04, Vol.32 (2), p.151-156
Main Authors: Ilse, Scheirlinck, Roel, Van der Meulen, Gino, Vrancken, Luc, De Vuyst, Luca, Settanni, Peter, Vandamme, Geert, Huys
Format: Article
Language:English
Subjects:
(GTG) 5-PCR
Bacterial Typing Techniques
Bacteriology
Belgium
Biological and medical sciences
Bread - microbiology
DNA Fingerprinting - methods
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
DNA-Directed RNA Polymerases - genetics
Fundamental and applied biological sciences. Psychology
Italy
Lactobacillus
Lactobacillus - classification
Lactobacillus - genetics
Lactobacillus - isolation & purification
Lactobacillus rossiae
Microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phenylalanine-tRNA Ligase - genetics
Phes
Polyphasic taxonomy
RNA, Ribosomal, 16S - genetics
Rpoa
Sequence Analysis, DNA
sourdough
Species Specificity
Systematics
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Summary:(GTG) 5-PCR fingerprinting and pheS sequence analysis of 18 Lactobacillus rossiae isolates, mainly originating from Belgian and Italian artisan sourdoughs, revealed intraspecies grouping as evidenced by the delineation of three and two subgroups, respectively. On the other hand, 16S rRNA and rpoA gene sequence analysis and DNA–DNA hybridizations supported the accommodation of all isolates in a single species. No correlation between genetic and phenotypic heterogeneity was observed. Collectively, these data do not warrant taxonomic division of L. rossiae. On the other hand, the considerable differences in intraspecies sequence variation of L. rossiae isolates displayed by the pheS (9.8%) and rpoA (1.1%) genes highlight that the discriminatory power of housekeeping genes as alternative genomic markers for the 16S rRNA gene in the identification of Lactobacillus species may significantly differ from gene to gene. In conclusion, this study has demonstrated that a polyphasic approach remains highly useful for identification of isolates belonging to genotypically heterogeneous species such as L. rossiae.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2008.12.006