Ror2 expression in squamous cell carcinoma and epithelial dysplasia of the oral cavity

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2009-03, Vol.107 (3), p.398-406
• Main Authors: Kobayashi, Masaki, DDS, Shibuya, Yasuyuki, DDS, PhD, Takeuchi, Junichiro, DDS, Murata, Maho, DDS, PhD, Suzuki, Hiroaki, DDS, PhD, Yokoo, Satoshi, DDS, PhD, Umeda, Masahiro, DDS, PhD, Minami, Yasuhiro, MD, PhD, Komori, Takahide, DDS, PhD
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Blotting, Western; Carcinoma, Squamous Cell - chemistry; Carcinoma, Squamous Cell - metabolism; Case-Control Studies; Cell Membrane - chemistry; Dentistry; Dermatology; Endoplasmic Reticulum, Rough - chemistry; Female; Gene Expression; Humans; Immunohistochemistry; Ki-67 Antigen - biosynthesis; Male; Medical sciences; Middle Aged; Otorhinolaryngology. Stomatology; Precancerous Conditions - chemistry; Precancerous Conditions - metabolism; Proliferating Cell Nuclear Antigen - biosynthesis; Receptor Protein-Tyrosine Kinases - biosynthesis; Receptor Tyrosine Kinase-like Orphan Receptors; Receptors, Cell Surface - biosynthesis; Surgery; Tongue Neoplasms - chemistry; Tongue Neoplasms - metabolism; Tumor Suppressor Protein p53 - biosynthesis; Tumors of the skin and soft tissue. Premalignant lesions
• ISSN: 1079-2104; 1528-395X
• DOI: 10.1016/j.tripleo.2008.08.018

In this study, the expressions of Ror2 in the normal mucosa, the epithelium dysplasia, and squamous cell carcinoma (SCC) of the oral cavity were investigated, and possible differences in the expression patterns of Ror2 and of p53, Ki67, or PCNA were examined. In Western blotting analyses, Ror2 expression in oral cancer was significantly higher than that in the normal oral mucosa. Immunohistochemically, Ror2 was localized on the plasmalemma and in the rough endoplasmic reticulum (rER). The tissue area with an Ror2-positive expression tended to differ from the area with a positive expression of p53, ki67, or PCNA, and the number of cells with an Ror2 expression tended to increase as the degree of malignancy rose in the epithelial tissues. These results suggest that Ror2 was not related to cell proliferation, but rather associated with cell polarity and cell motility, and that it was also closely associated with the degree of malignancy in oral epithelial tissue.