Encystment of Azotobacter nigricans grown diazotrophically on kerosene as sole carbon source

Encystment of Azotobacter nigricans was induced by its diazotrophic cultivation on kerosene. Its growth and nitrogenase activity were affected by kerosene in comparison to cultures grown on sucrose. Electron microscopy of vegetative cells showed that when nitrogenase activity was higher and the poly...

Full description

Saved in:
Bibliographic Details
Published in:Archives of microbiology 2009-03, Vol.191 (3), p.275-281
Main Authors: García-Esquivel, Gabriela, Calva-Calva, Graciano, Ferrera-Cerrato, Ronald, Fernández-Linares, Luis Carlos, Vázquez, Refugio Rodríguez, Esparza-García, Fernando José
Format: Article
Language:English
Subjects:
Alcohol
Azotobacter - cytology
Azotobacter - growth & development
Azotobacter - isolation & purification
Azotobacter - metabolism
Bacteriology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carbon
Carbon - metabolism
Carbon sources
Cell Biology
Cell division
Cysts
Ecology
Fundamental and applied biological sciences. Psychology
Hydroxybutyrates - analysis
Kerosene
Life Sciences
Medical sciences
Microbial Ecology
Microbiology
Microscopy
Miscellaneous
Nitrogenase - metabolism
Polyesters - analysis
Short Communication
Soil contamination
Spores, Bacterial - metabolism
Spores, Bacterial - ultrastructure
Sucrose
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Encystment of Azotobacter nigricans was induced by its diazotrophic cultivation on kerosene. Its growth and nitrogenase activity were affected by kerosene in comparison to cultures grown on sucrose. Electron microscopy of vegetative cells showed that when nitrogenase activity was higher and the poly-β-hydroxybutyrate granules were not present to a significant extent, peripheral bodies were abundant. After 8 days of culture on kerosene, the presence of cysts with intracellular bunches of poly-β-hydroxybutyrate granules was observed. Germination of cysts bears germinating multicelled yet unbroken capsule cysts with up to three cells inside. This is the first report of encystment induction of Azotobacter species grown on kerosene.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-008-0444-9