Saucernetin-8 Isolated from Saururus chinensis Induced the Differentiation of Human Acute Promyelocytic Leukemia HL-60 Cells

The present work was performed to investigate the effects of saucernetin-8 on proliferation and differentiation of human leukemia HL-60 cells as well as the underlying mechanisms for these effects. Saucernetin-8 exhibited a potent antiproliferative activity against HL-60 cells. This compound was als...

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Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2004, Vol.27(10), pp.1594-1598
Main Authors: Seo, Bo-Rim, Yoo, Chae-Bin, Park, Hee-Juhn, Choi, Jong-Won, Seo, Kyungwon, Choi, Seung-Ki, Lee, Kyung-Tae
Format: Article
Language:English
Subjects:
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
c-myc
Cell Cycle Proteins - biosynthesis
Cell Cycle Proteins - genetics
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Cyclin-Dependent Kinase Inhibitor p21
differentiation
Down-Regulation
Furans - isolation & purification
Furans - pharmacology
Genes, myc - drug effects
HL-60 Cells
Humans
Lignans - isolation & purification
Lignans - pharmacology
p21CIP1
Plant Extracts - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
saucernetin-8
Saururaceae
Up-Regulation
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Summary:The present work was performed to investigate the effects of saucernetin-8 on proliferation and differentiation of human leukemia HL-60 cells as well as the underlying mechanisms for these effects. Saucernetin-8 exhibited a potent antiproliferative activity against HL-60 cells. This compound was also found to be a potent inducer of differentiation in human leukemia derived HL-60 cells through the examination of differentiation markers, as assessed by nitroblue tetrazolium reduction test, esterase activity assay, phagocytic activity assay, morphology change, and expression of CD14 and CD66b surface antigens. These results suggest that saucernetin-8 induces the differentiation of human leukemia cells to granulocytes and monocytes/macrophages lineage. Moreover, DNA flow-cytometry indicated that saucernetin-8 induced a G1 phase arrest of HL-60 cells. The protein and mRNA expression levels of p21 were up-regulated during saucernetin-8-dependent HL-60 cell differentiation, whereas the level of c-myc was down-regulated. Taken together, our results suggest that saucernetin-8 may have potential as a therapeutic agent in human leukemia.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.27.1594