Relationship between Hpt polymorphisms and serum protein electropherogram

Serum protein electrophoresis is routinely used to identify pathologies involving dysproteinemia. The electropherogram mainly represents the most abundant serum proteins, one of which is the polymorphic haptoglobin (Hpt), characterized by a molecular heterogeneity with three major phenotypes (Hpt 1–...

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Bibliographic Details
Published in:Electrophoresis 2009-02, Vol.30 (3), p.525-531
Main Authors: Guerranti, Roberto, Bertocci, Erica, Muzzi, Chiara, Leoncini, Roberto, Rossi, Stefania, Scapellato, Carlo, Pagani, Roberto
Format: Article
Language:English
Subjects:
CZE
Electrophoresis, Capillary - methods
Haptoglobin
Haptoglobins - analysis
Haptoglobins - genetics
Phenotype
Polymorphism
Polymorphism, Genetic
Serum protein
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Summary:Serum protein electrophoresis is routinely used to identify pathologies involving dysproteinemia. The electropherogram mainly represents the most abundant serum proteins, one of which is the polymorphic haptoglobin (Hpt), characterized by a molecular heterogeneity with three major phenotypes (Hpt 1–1, 2–1, and 2–2). To improve the interpretation of electropherogram and possibly to extend its applicability, we aimed to explore the relationship between Hpt phenotypes (determined by immunoblotting) and protein profiles. Serum samples were separated by CZE with PROTEIN 6 and high‐resolution methods. The PROTEIN 6 analysis showed significant associations between α2 zone profiles and Hpt phenotypes (chi‐square=154.06, p
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200800366