Differential DNA methylation reprogramming of various repetitive sequences in mouse preimplantation embryos

Genome-wide changes of DNA methylation by active and passive demethylation processes are typical features during preimplantation development. Here we provide an insight that epigenetic reprogramming of DNA methylation is regulated in a region-specific manner, not a genome-wide fashion. To address th...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-11, Vol.324 (1), p.58-63
Main Authors: Kim, Seok-Ho, Kang, Yong-Kook, Koo, Deog-Bon, Kang, Man-Jong, Moon, Seung-Ju, Lee, Kyung-Kwang, Han, Yong-Mahn
Format: Article
Language:English
Subjects:
Animals
Blastocyst - physiology
DNA Methylation
Epigenesis, Genetic
Epigenetic reprogramming
Female
Gene Expression Regulation, Developmental
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mouse
Oocytes - physiology
Pregnancy
Preimplantation embryo
Repetitive sequences
Repetitive Sequences, Nucleic Acid - genetics
Spermatozoa - physiology
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Summary:Genome-wide changes of DNA methylation by active and passive demethylation processes are typical features during preimplantation development. Here we provide an insight that epigenetic reprogramming of DNA methylation is regulated in a region-specific manner, not a genome-wide fashion. To address this hypothesis, methylation states of three repetitive genomic regions were monitored at various developmental stages in the mouse embryos. Active demethylation was not observed in the IAP sequences whereas methylation reprogramming of the satellite sequences was regulated only by the active mechanism. Etn elements were actively demethylated after fertilization, passively demethylated by the 8-cell stage, and de novo methylated at the morular and blastocyst stages, showing dynamic epigenetic changes. Thus, our findings suggest that the specific genomic regions or sequences may spatially/temporally have their unique characteristics in the reprogramming of the DNA methylation during preimplantation development.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.09.023