Rapid, automated, nonradiometric susceptibility testing of Mycobacterium tuberculosis complex to four first-line antituberculous drugs used in standard short-course chemotherapy

The increasing prevalence of drug-resistant tuberculosis necessitates rapid and accurate susceptibility testing. The nonradiometric BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) system for susceptibility testing was evaluated on 222 clinical Mycobacterium tuberculosis complex isolates for iso...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2004-10, Vol.50 (2), p.103-107
Main Authors: Johansen, Isik Somuncu, Thomsen, Vibeke Østergaard, Marjamäki, Merja, Sosnovskaja, Anaida, Lundgren, Bettina
Format: Article
Language:English
Subjects:
Antimicrobial susceptibility test
Antitubercular Agents - pharmacology
BACTEC 460
BACTEC MGIT 960
Biological and medical sciences
Colony Count, Microbial
Culture Media
Drug Resistance, Multiple, Bacterial
Ethambutol - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Isoniazid - pharmacology
Medical sciences
Microbial Sensitivity Tests - instrumentation
Microbial Sensitivity Tests - methods
Microbiology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - growth & development
Pyrazinamide
Pyrazinamide - pharmacology
Rifampin - pharmacology
Sampling Studies
Sensitivity and Specificity
Tuberculosis, Multidrug-Resistant - diagnosis
Tuberculosis, Multidrug-Resistant - drug therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The increasing prevalence of drug-resistant tuberculosis necessitates rapid and accurate susceptibility testing. The nonradiometric BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) system for susceptibility testing was evaluated on 222 clinical Mycobacterium tuberculosis complex isolates for isoniazid, rifampin, and ethambutol. Fifty-seven of the isolates were tested for pyrazinamide. Results were compared to those of radiometric BACTEC 460 system and discrepancies were resolved by the agar proportion method. We found an overall agreement of 99.0% for isoniazid, 99.5% for rifampin, 98.2% for ethambutol, and 100% for pyrazinamide. After resolution of discrepancies, MGIT yielded no false susceptibility for rifampin and isoniazid. Although turnaround times were comparable, MGIT provides an advantage as inoculation can be done on any weekday as the growth is monitored automatically. The automated MGIT system is a rapid and reliable alternative for susceptibility testing of M. tuberculosis complex to first-line drugs.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2004.04.001