The influence of novel bioactive glasses on in vitro osteoblast behavior

Implant success requires a direct bond between bone and implant surface. Bioinert implants, such as titanium alloys, are commonly plasma‐spray–coated with a bone‐bonding, bioactive material such as hydroxyapatite. Such coatings tend to be chemically and topographically inhomogeneous without reproduc...

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Bibliographic Details
Published in:Journal of biomedical materials research 2004-11, Vol.71A (2), p.242-249
Main Authors: Foppiano, Silvia, Marshall, Sally J., Marshall, Grayson W., Saiz, Eduardo, Tomsia, Antoni P.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
bioactive glass
biocompatibility
Calcification, Physiologic - physiology
Cell Adhesion
Cell Line
Cell Proliferation
cell-material interactions
Glass - chemistry
hard tissue
Mice
Microscopy, Electron, Scanning
Osteoblasts - cytology
Osteoblasts - enzymology
Osteoblasts - physiology
Phosphates - chemistry
Phosphates - metabolism
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Summary:Implant success requires a direct bond between bone and implant surface. Bioinert implants, such as titanium alloys, are commonly plasma‐spray–coated with a bone‐bonding, bioactive material such as hydroxyapatite. Such coatings tend to be chemically and topographically inhomogeneous without reproducible properties. A family of bioactive glasses that can be enameled and reliably adheres to titanium alloy has been developed. In this study the cytocompatibility of two of these glass compositions was tested in the as‐cast condition. The effects of these glasses on the early and late events of osseous tissue formation in vitro were determined with MC3T3‐E1.4 mouse osteoblast‐like cells. MC3T3‐E1.4 cells were cultured on glasses containing 55 and 50 wt % SiO2, with titanium alloy (Ti6Al4V) and tissue culture polystyrene as controls. Cellular adhesion and proliferation, and alkaline phosphatase activity were studied over 5 to 15 days in culture. Qualitative and quantitative assays of mineralization were conducted. The osteoblast‐like cells showed increased proliferation when grown on a bioactive glass containing 50 wt % silica. However, the adhesion, differentiation and mineralization behavior were similar on both glass compositions used in this study. These bioactive glasses proved to be cytocompatible substrata for osteoblast‐like cell culture, and yielded higher cellular proliferation than titanium alloy. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 242–249, 2004
ISSN:1549-3296
0021-9304
1552-4965
DOI:10.1002/jbm.a.30159