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Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors
Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M 1–4 muscarinic receptor subtypes and α 2B -, β 2 -, and β 3 -adrenoceptors as...
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| Published in: | Naunyn-Schmiedeberg's archives of pharmacology 2009-04, Vol.379 (4), p.397-402 |
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| Main Authors: | , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c451t-f4d33c1373e854eb6e546326810a092f359bd6ffed16650238b0ea74bd365ada3 |
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| cites | cdi_FETCH-LOGICAL-c451t-f4d33c1373e854eb6e546326810a092f359bd6ffed16650238b0ea74bd365ada3 |
| container_end_page | 402 |
| container_issue | 4 |
| container_start_page | 397 |
| container_title | Naunyn-Schmiedeberg's archives of pharmacology |
| container_volume | 379 |
| creator | Pradidarcheep, Wisuit Stallen, Jan Labruyère, Wil T. Dabhoiwala, Noshir F. Michel, Martin C. Lamers, Wouter H. |
| description | Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M
1–4
muscarinic receptor subtypes and α
2B
-, β
2
-, and β
3
-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used. |
| doi_str_mv | 10.1007/s00210-009-0393-0 |
| format | article |
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1–4
muscarinic receptor subtypes and α
2B
-, β
2
-, and β
3
-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.</description><identifier>ISSN: 0028-1298</identifier><identifier>EISSN: 1432-1912</identifier><identifier>DOI: 10.1007/s00210-009-0393-0</identifier><identifier>PMID: 19198807</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Antibodies - immunology ; Antibody Specificity - immunology ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Brain Chemistry ; Cell Line ; Gastrointestinal Tract - chemistry ; Humans ; Immune Sera - immunology ; Immunohistochemistry ; Neurosciences ; Pharmacology/Toxicology ; Rats ; Rats, Wistar ; Receptors, Adrenergic - analysis ; Receptors, Adrenergic - genetics ; Receptors, Adrenergic - immunology ; Receptors, Muscarinic - analysis ; Receptors, Muscarinic - genetics ; Receptors, Muscarinic - immunology ; Special Article ; Transfection ; Urinary Tract - chemistry</subject><ispartof>Naunyn-Schmiedeberg's archives of pharmacology, 2009-04, Vol.379 (4), p.397-402</ispartof><rights>The Author(s) 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-f4d33c1373e854eb6e546326810a092f359bd6ffed16650238b0ea74bd365ada3</citedby><cites>FETCH-LOGICAL-c451t-f4d33c1373e854eb6e546326810a092f359bd6ffed16650238b0ea74bd365ada3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19198807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pradidarcheep, Wisuit</creatorcontrib><creatorcontrib>Stallen, Jan</creatorcontrib><creatorcontrib>Labruyère, Wil T.</creatorcontrib><creatorcontrib>Dabhoiwala, Noshir F.</creatorcontrib><creatorcontrib>Michel, Martin C.</creatorcontrib><creatorcontrib>Lamers, Wouter H.</creatorcontrib><title>Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors</title><title>Naunyn-Schmiedeberg's archives of pharmacology</title><addtitle>Naunyn-Schmied Arch Pharmacol</addtitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><description>Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M
1–4
muscarinic receptor subtypes and α
2B
-, β
2
-, and β
3
-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antibody Specificity - immunology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Brain Chemistry</subject><subject>Cell Line</subject><subject>Gastrointestinal Tract - chemistry</subject><subject>Humans</subject><subject>Immune Sera - immunology</subject><subject>Immunohistochemistry</subject><subject>Neurosciences</subject><subject>Pharmacology/Toxicology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Adrenergic - analysis</subject><subject>Receptors, Adrenergic - genetics</subject><subject>Receptors, Adrenergic - immunology</subject><subject>Receptors, Muscarinic - analysis</subject><subject>Receptors, Muscarinic - genetics</subject><subject>Receptors, Muscarinic - immunology</subject><subject>Special Article</subject><subject>Transfection</subject><subject>Urinary Tract - chemistry</subject><issn>0028-1298</issn><issn>1432-1912</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kM1LxDAQxYMo7rr6B3iRnrxVJ02btkcRv2DBi57DNJ0sWftl0gr735ulBW9eZni8Nw_mx9g1hzsOkN97gIRDDFDGIEoRwwlb81QkMS95csrWwS5inpTFil14vwcAybPsnK2CXxYF5GtGW9RfUW8iP5C2xmo7Ho5S921LTltsmkOEP2gbrBqKsButJ4cR7tB2fozayWt0tiO3szrYdYS1o0U60jSMvfOX7Mxg4-lq2Rv2-fz08fgab99f3h4ftrFOMz7GJq2F0FzkgoospUpSlkqRyIIDQpkYkZVVLY2hmkuZQSKKCgjztKqFzLBGsWG3c-_g-u-J_Kha6zU1DXbUT15JWaaCyzQE-RzUrvfekVGDsy26g-KgjmzVzFYFturINowNu1nKp6ql-u9igRkCyRzwwep25NS-n1wXHv6n9RcJvoW_</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Pradidarcheep, Wisuit</creator><creator>Stallen, Jan</creator><creator>Labruyère, Wil T.</creator><creator>Dabhoiwala, Noshir F.</creator><creator>Michel, Martin C.</creator><creator>Lamers, Wouter H.</creator><general>Springer-Verlag</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors</title><author>Pradidarcheep, Wisuit ; 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1–4
muscarinic receptor subtypes and α
2B
-, β
2
-, and β
3
-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19198807</pmid><doi>10.1007/s00210-009-0393-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0028-1298 |
| ispartof | Naunyn-Schmiedeberg's archives of pharmacology, 2009-04, Vol.379 (4), p.397-402 |
| issn | 0028-1298 1432-1912 |
| language | eng |
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| source | Springer Nature |
| subjects | Animals Antibodies - immunology Antibody Specificity - immunology Biomedical and Life Sciences Biomedicine Blotting, Western Brain Chemistry Cell Line Gastrointestinal Tract - chemistry Humans Immune Sera - immunology Immunohistochemistry Neurosciences Pharmacology/Toxicology Rats Rats, Wistar Receptors, Adrenergic - analysis Receptors, Adrenergic - genetics Receptors, Adrenergic - immunology Receptors, Muscarinic - analysis Receptors, Muscarinic - genetics Receptors, Muscarinic - immunology Special Article Transfection Urinary Tract - chemistry |
| title | Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors |
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