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Encapsulation and/or Release Behavior of Bovine Serum Albumin within and from Polylactide-Grafted Dextran Microspheres
Polylactide (PLA)‐grafted dextran (Dex‐graft‐PLA) of various contents of sugar units was synthesized by anionic polymerization of L‐lactide (L‐LA) using the alkoxide of partially trimethylsilylated dextran (TMSDex) and subsequently removing the trimethylsilyl (TMS) groups. The copolymer showed diffe...
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| Published in: | Macromolecular bioscience 2004-04, Vol.4 (4), p.458-463 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5066-e65e29ca4173384aca7d4939d9f17d22a5d31db0ddad01d9676119412ee9362e3 |
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| cites | cdi_FETCH-LOGICAL-c5066-e65e29ca4173384aca7d4939d9f17d22a5d31db0ddad01d9676119412ee9362e3 |
| container_end_page | 463 |
| container_issue | 4 |
| container_start_page | 458 |
| container_title | Macromolecular bioscience |
| container_volume | 4 |
| creator | Ouchi, Tatsuro Saito, Toshifumi Kontani, Tomohiro Ohya, Yuichi |
| description | Polylactide (PLA)‐grafted dextran (Dex‐graft‐PLA) of various contents of sugar units was synthesized by anionic polymerization of L‐lactide (L‐LA) using the alkoxide of partially trimethylsilylated dextran (TMSDex) and subsequently removing the trimethylsilyl (TMS) groups. The copolymer showed different solubility from L‐LA homopolymer with increasing the content of sugar units. We prepared bovine serum albumin (BSA)‐loaded microspheres (MS)s according to a water‐in‐oil‐in‐water emulsion‐solvent evaporation/extraction method using methylene chloride/DMSO as an organic cosolvent. MSs prepared from Dex‐graft‐PLA [MS(Dex‐graft‐PLA)s] exhibited higher loading efficiency of BSA than MSs prepared from PLLA [MS(PLLA)s]. The in vitro release rate of BSA from MS(Dex‐graft‐PLA) was faster than that from MS(PLLA). BSA released from MS(Dex‐graft‐PLA) maintained the secondary structure of native BSA to a great extent, compared with BSA released from MS(PLLA).
Confocal fluorescence images of the differential interference micrographs over the fluorescence images of MS(PLLA) and MS(Dex‐graft‐PLA). |
| doi_str_mv | 10.1002/mabi.200300106 |
| format | article |
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Confocal fluorescence images of the differential interference micrographs over the fluorescence images of MS(PLLA) and MS(Dex‐graft‐PLA).</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.200300106</identifier><identifier>PMID: 15468238</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Applied sciences ; Biological and medical sciences ; biopolymers ; Capsules ; Circular Dichroism ; Dextrans - chemical synthesis ; Dextrans - chemistry ; drug delivery systems ; Exact sciences and technology ; Fluorescein-5-isothiocyanate ; General pharmacology ; graft copolymers ; Hydrogels - chemistry ; Indicators and Reagents ; Medical sciences ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; microspheres ; Natural polymers ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; Polyesters - chemical synthesis ; Polyesters - chemistry ; polylactide ; Serum Albumin, Bovine - secretion ; Starch and polysaccharides</subject><ispartof>Macromolecular bioscience, 2004-04, Vol.4 (4), p.458-463</ispartof><rights>Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5066-e65e29ca4173384aca7d4939d9f17d22a5d31db0ddad01d9676119412ee9362e3</citedby><cites>FETCH-LOGICAL-c5066-e65e29ca4173384aca7d4939d9f17d22a5d31db0ddad01d9676119412ee9362e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15703004$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15468238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ouchi, Tatsuro</creatorcontrib><creatorcontrib>Saito, Toshifumi</creatorcontrib><creatorcontrib>Kontani, Tomohiro</creatorcontrib><creatorcontrib>Ohya, Yuichi</creatorcontrib><title>Encapsulation and/or Release Behavior of Bovine Serum Albumin within and from Polylactide-Grafted Dextran Microspheres</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>Polylactide (PLA)‐grafted dextran (Dex‐graft‐PLA) of various contents of sugar units was synthesized by anionic polymerization of L‐lactide (L‐LA) using the alkoxide of partially trimethylsilylated dextran (TMSDex) and subsequently removing the trimethylsilyl (TMS) groups. The copolymer showed different solubility from L‐LA homopolymer with increasing the content of sugar units. We prepared bovine serum albumin (BSA)‐loaded microspheres (MS)s according to a water‐in‐oil‐in‐water emulsion‐solvent evaporation/extraction method using methylene chloride/DMSO as an organic cosolvent. MSs prepared from Dex‐graft‐PLA [MS(Dex‐graft‐PLA)s] exhibited higher loading efficiency of BSA than MSs prepared from PLLA [MS(PLLA)s]. The in vitro release rate of BSA from MS(Dex‐graft‐PLA) was faster than that from MS(PLLA). BSA released from MS(Dex‐graft‐PLA) maintained the secondary structure of native BSA to a great extent, compared with BSA released from MS(PLLA).
Confocal fluorescence images of the differential interference micrographs over the fluorescence images of MS(PLLA) and MS(Dex‐graft‐PLA).</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>biopolymers</subject><subject>Capsules</subject><subject>Circular Dichroism</subject><subject>Dextrans - chemical synthesis</subject><subject>Dextrans - chemistry</subject><subject>drug delivery systems</subject><subject>Exact sciences and technology</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>General pharmacology</subject><subject>graft copolymers</subject><subject>Hydrogels - chemistry</subject><subject>Indicators and Reagents</subject><subject>Medical sciences</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron, Scanning</subject><subject>microspheres</subject><subject>Natural polymers</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>Polyesters - chemical synthesis</subject><subject>Polyesters - chemistry</subject><subject>polylactide</subject><subject>Serum Albumin, Bovine - secretion</subject><subject>Starch and polysaccharides</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkc9v0zAUxyMEYmNw5Yh8gVs6_0js-NiO0U1aAY0iuFmv8YtqcJLOTrr1v19Cq7LbTs96-ny_tr_fJHnP6IRRys9rWLkJp1RQyqh8kZwyyWSaM52_PJ4LdZK8ifHPgKhC89fJCcszWXBRnCbby6aETew9dK5tCDT2vA3kFj1CRDLDNWzdsGgrMmu3rkHyA0Nfk6lf9bVryL3r1u6fjFShrcn31u88lJ2zmM4DVB1a8hkfugANWbgytHGzxoDxbfKqAh_x3WGeJT-_XC4vrtKbb_Pri-lNWuZUyhRljlyXkDElRJFBCcpmWmirK6Ys55BbweyKWguWMqulkozpjHFELSRHcZZ82vtuQnvXY-xM7WKJ3kODbR-NlDoTispnQU6LITE1gpM9OH4mBqzMJrgaws4wasZKzFiJOVYyCD4cnPtVjfY_fuhgAD4eAIgl-GrIqnTxCadGq2zg9J67dx53z1xrFtPZ9dNHpHutix0-HLUQ_hqphMrNr69z83spZ7dZtjQL8Qi0mbTK</recordid><startdate>20040419</startdate><enddate>20040419</enddate><creator>Ouchi, Tatsuro</creator><creator>Saito, Toshifumi</creator><creator>Kontani, Tomohiro</creator><creator>Ohya, Yuichi</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040419</creationdate><title>Encapsulation and/or Release Behavior of Bovine Serum Albumin within and from Polylactide-Grafted Dextran Microspheres</title><author>Ouchi, Tatsuro ; Saito, Toshifumi ; Kontani, Tomohiro ; Ohya, Yuichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5066-e65e29ca4173384aca7d4939d9f17d22a5d31db0ddad01d9676119412ee9362e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>biopolymers</topic><topic>Capsules</topic><topic>Circular Dichroism</topic><topic>Dextrans - chemical synthesis</topic><topic>Dextrans - chemistry</topic><topic>drug delivery systems</topic><topic>Exact sciences and technology</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>General pharmacology</topic><topic>graft copolymers</topic><topic>Hydrogels - chemistry</topic><topic>Indicators and Reagents</topic><topic>Medical sciences</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Electron, Scanning</topic><topic>microspheres</topic><topic>Natural polymers</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>Polyesters - chemical synthesis</topic><topic>Polyesters - chemistry</topic><topic>polylactide</topic><topic>Serum Albumin, Bovine - secretion</topic><topic>Starch and polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ouchi, Tatsuro</creatorcontrib><creatorcontrib>Saito, Toshifumi</creatorcontrib><creatorcontrib>Kontani, Tomohiro</creatorcontrib><creatorcontrib>Ohya, Yuichi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ouchi, Tatsuro</au><au>Saito, Toshifumi</au><au>Kontani, Tomohiro</au><au>Ohya, Yuichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Encapsulation and/or Release Behavior of Bovine Serum Albumin within and from Polylactide-Grafted Dextran Microspheres</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2004-04-19</date><risdate>2004</risdate><volume>4</volume><issue>4</issue><spage>458</spage><epage>463</epage><pages>458-463</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>Polylactide (PLA)‐grafted dextran (Dex‐graft‐PLA) of various contents of sugar units was synthesized by anionic polymerization of L‐lactide (L‐LA) using the alkoxide of partially trimethylsilylated dextran (TMSDex) and subsequently removing the trimethylsilyl (TMS) groups. The copolymer showed different solubility from L‐LA homopolymer with increasing the content of sugar units. We prepared bovine serum albumin (BSA)‐loaded microspheres (MS)s according to a water‐in‐oil‐in‐water emulsion‐solvent evaporation/extraction method using methylene chloride/DMSO as an organic cosolvent. MSs prepared from Dex‐graft‐PLA [MS(Dex‐graft‐PLA)s] exhibited higher loading efficiency of BSA than MSs prepared from PLLA [MS(PLLA)s]. The in vitro release rate of BSA from MS(Dex‐graft‐PLA) was faster than that from MS(PLLA). BSA released from MS(Dex‐graft‐PLA) maintained the secondary structure of native BSA to a great extent, compared with BSA released from MS(PLLA).
Confocal fluorescence images of the differential interference micrographs over the fluorescence images of MS(PLLA) and MS(Dex‐graft‐PLA).</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>15468238</pmid><doi>10.1002/mabi.200300106</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Macromolecular bioscience, 2004-04, Vol.4 (4), p.458-463 |
| issn | 1616-5187 1616-5195 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Applied sciences Biological and medical sciences biopolymers Capsules Circular Dichroism Dextrans - chemical synthesis Dextrans - chemistry drug delivery systems Exact sciences and technology Fluorescein-5-isothiocyanate General pharmacology graft copolymers Hydrogels - chemistry Indicators and Reagents Medical sciences Microscopy, Confocal Microscopy, Electron, Scanning microspheres Natural polymers Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Physicochemistry of polymers Polyesters - chemical synthesis Polyesters - chemistry polylactide Serum Albumin, Bovine - secretion Starch and polysaccharides |
| title | Encapsulation and/or Release Behavior of Bovine Serum Albumin within and from Polylactide-Grafted Dextran Microspheres |
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