Vasopressin regulation of the renal UT-A3 urea transporter

Facilitative urea transporters in the mammalian kidney play a vital role in the urinary concentrating mechanism. The urea transporters located in the renal inner medullary collecting duct, namely UT-A1 and UT-A3, are acutely regulated by the antidiuretic hormone vasopressin. In this study, we invest...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Renal physiology 2009-03, Vol.296 (3), p.F642-F648
Main Authors: Stewart, G S, Thistlethwaite, A, Lees, H, Cooper, G J, Smith, Craig
Format: Article
Language:English
Subjects:
Animals
Casein Kinase II - metabolism
Cell Line
Cells
Dogs
Kidney - metabolism
Kidneys
Kinases
Mammals
Membrane Transport Proteins - metabolism
Membranes
Studies
Urea - metabolism
Urea Transporters
Vasopressins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Facilitative urea transporters in the mammalian kidney play a vital role in the urinary concentrating mechanism. The urea transporters located in the renal inner medullary collecting duct, namely UT-A1 and UT-A3, are acutely regulated by the antidiuretic hormone vasopressin. In this study, we investigated the vasopressin regulation of the basolateral urea transporter UT-A3 using an MDCK-mUT-A3 cell line. Within 10 min, vasopressin stimulates urea flux through UT-A3 transporters already present at the plasma membrane, via a PKA-dependent process. Within 1 h, vasopressin significantly increases UT-A3 localization at the basolateral membrane, causing a further increase in urea transport. While the basic trafficking of UT-A3 to basolateral membranes involves both protein kinase C and calmodulin, its regulation by vasopressin specifically occurs through a casein kinase II-dependent pathway. In conclusion, this study details the effects of vasopressin on UT-A3 urea transporter function and hence its role in regulating urea permeability within the renal inner medullary collecting duct.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.90660.2008