The first detection of Cryptosporidium deer-like genotype in cattle in Japan

• Published in: Parasitology research (1987) 2009-03, Vol.104 (4), p.745-752
• Main Authors: Amer, Said, Honma, Hajime, Ikarashi, Makoto, Oishi, Ryu, Endo, Mikiko, Otawa, Kenichi, Nakai, Yutaka
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - parasitology; Cryptosporidiosis - epidemiology; Cryptosporidiosis - parasitology; Cryptosporidiosis - veterinary; Cryptosporidium - classification; Cryptosporidium - genetics; Cryptosporidium - isolation & purification; Dairying; Deer; DNA, Protozoan - analysis; DNA, Protozoan - isolation & purification; Feces - parasitology; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Genes, rRNA; Genotype; Immunology; Invertebrates; Japan - epidemiology; Medical Microbiology; Microbiology; Molecular Sequence Data; Original Paper; Phylogeny; Polymerase Chain Reaction - methods; RNA, Ribosomal, 18S - genetics; Sequence Analysis, DNA
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-008-1250-7

The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C . parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle from some farms around Osaki area, Miyagi prefecture, Japan, was examined on a field visit during August, 2007, by polymerase chain reaction techniques for detection, genotyping, and subtyping. Cryptosporidium oocysts were detected in the feces of five out of 50 animals. Of the five Cryptosporidium -positive specimens available for molecular analysis, C . parvum was identified in three specimens, Cryptosporidium deer-like genotype in one, and Cryptosporidium andersoni in one specimen. Amplification of Cpgp60 from C . andersoni and Cryptosporidium deer-like genotype samples revealed that these samples have light concurrent C . parvum infection. Sequence analysis of the 60-kDa glycoprotein gene indicated that all C . parvum samples are IIa subtype. Detection of Cryptosporidium deer-like genotype is geographically unique in Japan. The genetic diversity of Cryptosporidium in dairy cattle in Japan may be much greater than that reported before.