Ginsenoside compound K production from Ginseng [Panax pseudoginseng] root extract by a thermostable beta-glycosidase from Sulfolobus solfataricus

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-02, Vol.73 (2), p.316-321
• Main Authors: Noh, K.H.(Konkuk Univ., Seoul (Korea R.)), Son, J.W, Kim, H.J, Oh, D.K
• Format: Article
• Language: English
• Subjects: BACTERIA; Biological and medical sciences; biotransformation; CHEMICOPHYSICAL PROPERTIES; Enzyme Stability; EXTRACTOS VEGETALES; EXTRAIT D'ORIGINE VEGETALE; Fundamental and applied biological sciences. Psychology; GINSENOSIDE; ginsenoside compound K; GINSENOSIDES; GINSENOSIDOS; GLICOSIDASAS; Glucosidases - chemistry; Glucosidases - metabolism; GLYCOSIDASE; GLYCOSIDASES; HEAT TOLERANCE; Hydrogen-Ion Concentration; Panax - chemistry; PANAX PSEUDOGINSENG; PLANT EXTRACTS; Plant Extracts - chemistry; Plant Extracts - metabolism; Plant Roots - chemistry; PROPIEDADES FISICOQUIMICAS; PROPRIETE PHYSICOCHIMIQUE; RACINE; RAICES; ROOTS; Sapogenins - metabolism; Substrate Specificity; Sulfolobus solfataricus; Sulfolobus solfataricus - enzymology; Temperature; TOLERANCE A LA CHALEUR; TOLERANCIA AL CALOR; β-glycosidase
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80525

Ginsenoside compound K was produced from ginseng root extract using a thermostable recombinant β-glycosidase from Sulfolobus solfataricus. The pH and temperature for maximum production were 5.5 and 90 °C. The half-lives of the enzyme were 66, 30, and 1.7 h at 80, 85, and 90 °C respectively. The ginsenoside-hydrolyzing activity (Rd>Rb 1 >Rc>Rb 2 ) and compound K-producing activity (Rd>Rc>Rb 1 >Rb 2 ) of the β-glycosidase were also determined. At pH 5.5 at 85 °C, 1.63 mg/ml of compound K was produced within 12 h by 40 U/ml of enzyme from 1.9 mg/ml of ginsenoside Rb 1 , 0.52 mg/ml of ginsenoside Rb 2 , 0.92 mg/ml of ginsenoside Rc, and 0.23 mg/ml of ginsenoside Rd in a 10% (w/v) ginseng root extract via two transformation pathways, Rb 1 or Rb 2 → Rd → F 2 → compound K, and Rc → compound Mc → compound K.