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Role of Pir1 in the construction of the Candida albicans cell wall
Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Vicent Andrés Estelles s/n, 46100 Burjassot, València, Spain Correspondence Rafael Sentandreu Rafael.sentandreu{at}uv.es Searches in a Candida albicans database ( http://genolist.pasteur.fr/CandidaDB/ ) identifie...
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| Published in: | Microbiology (Society for General Microbiology) 2004-10, Vol.150 (10), p.3151-3161 |
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| creator | Martinez, Ana I Castillo, Luis Garcera, Ana Elorza, Maria V Valentin, Eulogio Sentandreu, Rafael |
| description | Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Vicent Andrés Estelles s/n, 46100 Burjassot, València, Spain
Correspondence Rafael Sentandreu Rafael.sentandreu{at}uv.es
Searches in a Candida albicans database ( http://genolist.pasteur.fr/CandidaDB/ ) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42 % and 45 % homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene ( CaPIR1 ) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O -glycosylated and has an N -mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells and are hypersensitive to drugs that affect cell wall assembly. CaPir1 was labelled with the V5 epitope and found linked to the 1,3- -glucan of the C. albicans wall and also by disulphide bridges when expressed in S. cerevisiae .
Abbreviations: ConA, concanavalin A; Endo-F, endoglycosidase F; IPF, individual protein file |
| doi_str_mv | 10.1099/mic.0.27220-0 |
| format | article |
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Correspondence Rafael Sentandreu Rafael.sentandreu{at}uv.es
Searches in a Candida albicans database ( http://genolist.pasteur.fr/CandidaDB/ ) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42 % and 45 % homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene ( CaPIR1 ) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O -glycosylated and has an N -mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells and are hypersensitive to drugs that affect cell wall assembly. CaPir1 was labelled with the V5 epitope and found linked to the 1,3- -glucan of the C. albicans wall and also by disulphide bridges when expressed in S. cerevisiae .
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Correspondence Rafael Sentandreu Rafael.sentandreu{at}uv.es
Searches in a Candida albicans database ( http://genolist.pasteur.fr/CandidaDB/ ) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42 % and 45 % homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene ( CaPIR1 ) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O -glycosylated and has an N -mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells and are hypersensitive to drugs that affect cell wall assembly. CaPir1 was labelled with the V5 epitope and found linked to the 1,3- -glucan of the C. albicans wall and also by disulphide bridges when expressed in S. cerevisiae .
Abbreviations: ConA, concanavalin A; Endo-F, endoglycosidase F; IPF, individual protein file</description><subject>Amino Acid Sequence</subject><subject>beta-Glucans - metabolism</subject><subject>Candida albicans - genetics</subject><subject>Candida albicans - physiology</subject><subject>Candida albicans - ultrastructure</subject><subject>Cell Wall - genetics</subject><subject>Cell Wall - physiology</subject><subject>Fungal Proteins - physiology</subject><subject>Glycoproteins</subject><subject>Saccharomyces cerevisiae Proteins - physiology</subject><subject>Sequence Homology, Amino Acid</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpFkE1LAzEQhoMotlaPXiUnD8LWyeZjd49a_IKCInoO2STbRrabmuxS_PdmbcHTDDPPPAwvQpcE5gSq6nbj9BzmeZHnkMERmhImeJZDCceppzwNyyKfoLMYvwDSEsgpmhDOCoBKTNH9u28t9g1-c4Fg1-F-bbH2XezDoHvnu3E3zhaqM84orNraadVFrG3b4p1q23N00qg22otDnaHPx4ePxXO2fH16WdwtM00F7TMDlooKCsMYhUKBIA2tLQMjSl03yjS80AUtRa0ULZuyLjiznBMwTFnNoaEzdL33boP_Hmzs5cbF8QvVWT9EKUTFyqqiCcz2oA4-xmAbuQ1uo8KPJCDH0NKhliD_QpOQ-KuDeKg31vzTh5QScLMH1m613rlg5cp2yRF87fwoI3wUS0o4ob-SZnU8</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Martinez, Ana I</creator><creator>Castillo, Luis</creator><creator>Garcera, Ana</creator><creator>Elorza, Maria V</creator><creator>Valentin, Eulogio</creator><creator>Sentandreu, Rafael</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Role of Pir1 in the construction of the Candida albicans cell wall</title><author>Martinez, Ana I ; Castillo, Luis ; Garcera, Ana ; Elorza, Maria V ; Valentin, Eulogio ; Sentandreu, Rafael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-d0e36907d44307a061f3be40d68cbfadf57c7386baa38f8b754e5510d4aec50f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>beta-Glucans - metabolism</topic><topic>Candida albicans - genetics</topic><topic>Candida albicans - physiology</topic><topic>Candida albicans - ultrastructure</topic><topic>Cell Wall - genetics</topic><topic>Cell Wall - physiology</topic><topic>Fungal Proteins - physiology</topic><topic>Glycoproteins</topic><topic>Saccharomyces cerevisiae Proteins - physiology</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez, Ana I</creatorcontrib><creatorcontrib>Castillo, Luis</creatorcontrib><creatorcontrib>Garcera, Ana</creatorcontrib><creatorcontrib>Elorza, Maria V</creatorcontrib><creatorcontrib>Valentin, Eulogio</creatorcontrib><creatorcontrib>Sentandreu, Rafael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez, Ana I</au><au>Castillo, Luis</au><au>Garcera, Ana</au><au>Elorza, Maria V</au><au>Valentin, Eulogio</au><au>Sentandreu, Rafael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Pir1 in the construction of the Candida albicans cell wall</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>150</volume><issue>10</issue><spage>3151</spage><epage>3161</epage><pages>3151-3161</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Vicent Andrés Estelles s/n, 46100 Burjassot, València, Spain
Correspondence Rafael Sentandreu Rafael.sentandreu{at}uv.es
Searches in a Candida albicans database ( http://genolist.pasteur.fr/CandidaDB/ ) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42 % and 45 % homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene ( CaPIR1 ) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O -glycosylated and has an N -mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells and are hypersensitive to drugs that affect cell wall assembly. CaPir1 was labelled with the V5 epitope and found linked to the 1,3- -glucan of the C. albicans wall and also by disulphide bridges when expressed in S. cerevisiae .
Abbreviations: ConA, concanavalin A; Endo-F, endoglycosidase F; IPF, individual protein file</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>15470096</pmid><doi>10.1099/mic.0.27220-0</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Alma/SFX Local Collection |
| subjects | Amino Acid Sequence beta-Glucans - metabolism Candida albicans - genetics Candida albicans - physiology Candida albicans - ultrastructure Cell Wall - genetics Cell Wall - physiology Fungal Proteins - physiology Glycoproteins Saccharomyces cerevisiae Proteins - physiology Sequence Homology, Amino Acid |
| title | Role of Pir1 in the construction of the Candida albicans cell wall |
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