The genes encoding cAMP-dependent protein kinase catalytic subunit homologues of the microsporidia Encephalitozoon intestinalis and E. cuniculi: molecular characterisation and phylogenetic analysis

A gene encoding a protein kinase was identified by homology-based PCR amplification in Encephalitozoon intestinalis, a microsporidian parasite pathogenic to humans, and its orthologue has been identified by database mining in the genome of the related species E. cuniculi, whose sequence has been rec...

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Bibliographic Details
Published in:Parasitology international 2004-12, Vol.53 (4), p.277-285
Main Authors: Equinet, Leila, Bapteste, Eric, Thellier, Marc, Ouarzane-Amara, Meryem, Vivarès, Christian P., Desportes-Livage, Isabelle, Doerig, Christian
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Catalytic Domain - genetics
Cell Line
Cloning, Molecular
Cyclic AMP-Dependent Protein Kinases - genetics
Cyclic AMP-Dependent Protein Kinases - metabolism
Encephalitozoon - enzymology
Encephalitozoon - genetics
Encephalitozoon - pathogenicity
Encephalitozoon cuniculi
Encephalitozoon cuniculi - enzymology
Encephalitozoon cuniculi - genetics
Encephalitozoon intestinalis
Genes, Protozoan
Microsporidia
Molecular Sequence Data
Phosphorylation
Phylogeny
Protein kinase
Protein kinase A
Rabbits
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:A gene encoding a protein kinase was identified by homology-based PCR amplification in Encephalitozoon intestinalis, a microsporidian parasite pathogenic to humans, and its orthologue has been identified by database mining in the genome of the related species E. cuniculi, whose sequence has been recently published. Phylogenetic analysis revealed that the proteins encoded by these genes are homologues of the cAMP-dependent protein kinase catalytic subunits (PKAc). Southern blot analysis indicated that the EiPKAc gene is present in two copies in the E. intestinalis genome, whereas the E. cuniculi orthologue ( EcPKAc) is a single copy gene. RT-PCR data showed that the EiPKAc gene is expressed in at least one of the intracellular stages during infection of the mammalian host cell by E. intestinalis.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2004.03.001