Stimulation of the rat medullary raphe nuclei induces differential responses in respiratory muscle activity

• Published in: Respiratory physiology & neurobiology 2009-02, Vol.165 (2), p.208-214
• Main Authors: Besnard, S, Denise, P, Cappelin, B, Dutschmann, M, Gestreau, C
• Format: Article
• Language: English
• Subjects: Anesthesia; Animals; Apnea; Biological and medical sciences; Electric Stimulation; Fundamental and applied biological sciences. Psychology; Male; Medical Education; Medulla Oblongata - cytology; Medulla Oblongata - physiology; Medullary raphe; Motor Neurons - physiology; Neural Inhibition - physiology; Neural Pathways; Pulmonary/Respiratory; Raphe Nuclei - cytology; Raphe Nuclei - physiology; Rats; Rats, Sprague-Dawley; Respiratory Mechanics - physiology; Respiratory Muscles - innervation; Respiratory Muscles - physiology; Serotonin; Sleep; Spinal Cord - cytology; Spinal Cord - physiology; Stimulation; Vertebrates: respiratory system
• ISSN: 1569-9048; 1878-1519
• DOI: 10.1016/j.resp.2008.12.004

Abstract Neural control circuits that coordinate the motor activity of the diaphragm (DIA) and the geniohyoid muscle (GH) are potentially involved in pathological conditions such as various forms of sleep apnea. Here we investigated a differential role of the raphe magnus (RMg), pallidus (RPa) and the obscurus (ROb) nuclei in the neural control of DIA and GH muscle activity in rats under volatile anesthesia. In order to characterize a topographical organization of the raphe nuclei we analyzed changes in DIA and GH during high-frequency stimulation (HFS, 10–130 Hz, 60 μs pulse width, 40–160 μA, 30 s). HFS of the RMg and the ROb induced apnea, in the latter case apnea was associated with massive tonic discharge in the GH. By contrast, HFS of the RPa induced tachypnea. At caudal stimulation sites the tachypnea was accompanied by tonic DIA activity and cessation of GH. These data suggest a differential distribution of inhibitory and excitatory drives of DIA and GH muscles within distinct raphe nuclei.