Rheological, sensorial, and chemopreventive properties of milk fermented with exopolysaccharide-producing lactic cultures

• Published in: Journal of dairy science 2009-03, Vol.92 (3), p.847-856
• Main Authors: Purohit, D.H, Hassan, A.N, Bhatia, E, Zhang, X, Dwivedi, C
• Format: Article
• Language: English
• Subjects: animal models; Animal productions; Animals; Biological and medical sciences; carcinogenesis; carcinogens; chemoprevention; Colon - enzymology; Colonic Neoplasms - prevention & control; colorectal neoplasms; Cultured Milk Products - standards; Cyclooxygenase 2 - metabolism; Diet; dietary supplements; exopolysaccharides; fermented milk; Food industries; Fundamental and applied biological sciences. Psychology; Gram-Positive Bacteria; Humans; Intestinal Neoplasms - prevention & control; lactic acid bacteria; lactic fermentation; Lactobacillus; Male; Milk and cheese industries. Ice creams; polysaccharides; Rats; rheological properties; Rheology; Sensation; sensory properties; starter cultures; Terrestrial animal productions; texture; Vertebrates
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2008-1256

The objective of this research was to evaluate the rheological, sensorial, and chemopreventive properties of milk fermented with different exopolysaccharide (EPS)-producing lactic cultures. Reconstituted skim milk (11% wt/vol) was fermented with single strains of EPS-producing and non-EPS-producing cultures. Whey that collected on the surface of undisturbed fermented milks and after cutting was measured. All EPS-producing cultures reduced the amount of whey present on the surface of the undisturbed samples, whereas only 3 out of 5 strains reduced syneresis measured after cutting. All EPS-producing cultures except a strain of Lactobacillus delbrueckii ssp. bulgaricus reduced viscoelastic moduli in fermented milk. There was a linear correlation between ropiness and smoothness. In the chemoprevention study, 140 male Fisher rats were divided into 7 groups of 20 each. Rats in 6 groups were fed diets supplemented with fermented milks each made with a single strain of EPS-producing or non-producing cultures, whereas rats in group 7 (control) were fed a diet supplemented with milk acidified with glucono-δ-lactone (GDL). All rats were injected with azoxymethane (15 mg/kg, subcutaneous) at wk 7 and 8 of age to induce tumors and fed their respective diets ad libitum throughout the study. After 30 wk of initiation, all rats were anesthetized with ether, and their intestinal tissues were isolated and washed with cold normal saline. The number and size of tumors in the colon and small intestine were recorded. Rats fed diets supplemented with fermented milk made with 2 EPS-positive and 1 EPS-negative strains had significantly lowered incidence of colon tumor and colon tumor multiplicity. Cyclooxygenase-2 enzyme activity (the enzyme implicated in colon tumor development) was significantly lower in the colon tissue of rats fed diets containing milk fermented with 4 EPS-producing and 1 non-producing cultures than that in rats fed diets supplemented with GDL-acidified milk. Different EPS-positive cultures produced fermented milks with distinct rheological characteristics and levels of ropiness. No relationship was found between rheological properties or level of ropiness of fermented milk and its chemopreventive effect.