Identification of a novel aminergic-like G protein-coupled receptor in the cnidarian Renilla koellikeri

• Published in: Gene 2004-10, Vol.341 (C), p.67-75
• Main Authors: Bouchard, Christelle, Ribeiro, Paula, Dubé, François, Demers, Christian, Anctil, Michel
• Format: Article
• Language: English
• Subjects: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Biogenic amines; Blotting, Northern; Cloning; Cloning, Molecular; Cnidaria; DNA, Complementary - chemistry; DNA, Complementary - genetics; Evolution; GPCR; In Situ Hybridization; Molecular Sequence Data; Receptors, G-Protein-Coupled - genetics; Renilla - genetics; Renilla koellikeri; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2004.07.020

Biogenic amines exert various physiological effects in cnidarians, but the receptors involved in these responses are not known. We have cloned a novel G protein-coupled receptor cDNA from an anthozoan, the sea pansy Renilla koellikeri, that shows homology to mammalian catecholamine receptors and, to a lesser extent, to peptidergic receptors. This putative receptor, named Ren2, has a DRC pattern that replaces the well-conserved DRY motif on the cytoplasmic side of the transmembrane III and lacks the cysteine residues usually found in the second extracellular loop and C-terminus tail. Both the second extracellular loop and the N-terminal tail were seen to be short (six and three amino acids, respectively). Northern blot analysis suggests that the receptor gene codes for two transcripts. Localization of these transcripts by in situ hybridization demonstrated abundant expression in the epithelium of the pharyngeal wall, the oral disk and tentacles as well as in the endodermal epithelium lining the gastrovascular cavities.